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Theoretical study of interactions of BSA protein in a NaCl aqueous solution

2013; American Institute of Physics; Volume: 138; Issue: 11 Linguagem: Inglês

10.1063/1.4794919

1520-9032

Giuseppe Pellicane, Miguel Cavero,

Spectroscopy and Quantum Chemical Studies

Bovine Serum Albumine (BSA) aqueous solutions in the presence of NaCl are investigated for different protein concentrations and low to intermediate ionic strengths. Protein interactions are modeled via a charge-screened colloidal model, in which the range of the potential is determined by the Debye-Hückel constant. We use Monte Carlo computer simulations to calculate the structure factor, and assume an oblate ellipsoidal form factor for BSA. The theoretical scattered intensities are found in good agreement with the experimental small angle X-ray scattering intensities available in the literature. The performance of well-known integral equation closures to the Ornstein-Zernike equation, namely the mean spherical approximation, the Percus-Yevick, and the hypernetted chain equations, is also assessed with respect to computer simulation.