Artigo Revisado por pares

Nucleic acid base-pairing and N-methylacetamide self-association in chloroform: affinity and conformation

1999; Elsevier BV; Volume: 78; Issue: 1-2 Linguagem: Inglês

10.1016/s0301-4622(98)00229-4

ISSN

1873-4200

Autores

Ray Luo, Martha S. Head, James A. Given, Michael K. Gilson,

Tópico(s)

Protein Structure and Dynamics

Resumo

A recently developed computational method, `mining minima', is used to examine the hydrogen-bonding interactions of nucleic acid base-pairs and of the N-methylacetamide homodimer in chloroform. The mining minima algorithm aggressively samples molecular conformations, identifies the most important local minima, and computes their contributions to the overall free energy of the system. Here, the CHARMM 98 parameter set is used for the potential energy and the generalized Born/surface area solvent model is used to account for the influence of the solvent. Good agreement with experiment is obtained for the non-covalent binding affinities of a series of complexes. The computational approach used here is applicable to a range of molecular systems.

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