Direct injection HPLC method for the determination of selected benzodiazepines in plasma using a Hisep column
2003; Elsevier BV; Volume: 33; Issue: 5 Linguagem: Inglês
10.1016/s0731-7085(03)00426-6
ISSN1873-264X
AutoresConstantinos Pistos, James T. Stewart,
Tópico(s)Molecular Sensors and Ion Detection
ResumoA direct plasma injection HPLC method has been developed for the determination of selected benzodiazepines (nitrazepam, clobazam, oxazepam, lorazepam). The method uses an analytical hydrophobic shielded phase (Hisep) column equipped with a Hisep guard column, are easy to perform and requires 20 ul of a filtered plasma sample. The chromatographic run time is less than 15 min using a mobile phase of 15:85 v/v acetonitrile–0.18 M ammonium acetate pH 2.5. The method is good for 175 injections before replacement of the guard column. The method was linear in the range 0.5–18 ug ml−1 (r>0.99, n=6) for the analytes with R.S.D. less than 10.82%. Interday and intraday variability were found to be less than 14%. The limits of detection and quantitation were 0.16 (s/n>3) and 0.5 ug ml−1 (s/n>10), respectively, for each of the four benzodiazepines.
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