Biotin-Avidin Amplified Elisa for Quantitation of Human IgA

1985; Marcel Dekker; Volume: 6; Issue: 1-2 Linguagem: Inglês

10.1080/01971528508063021

ISSN

2332-4090

Autores

Karen Adler‐Storthz, Gordon R. Dreesman, David Y. Graham, D. Gareth Evans,

Tópico(s)

Advanced Biosensing Techniques and Applications

Resumo

Abstract A biotin-avidin amplified enzyme-linked immunosorbent assay (ELISA) for the measurement of human IgA in serum, colostrum, gastric juice, and supernatants of in vitro mitogen-stimulated human peripheral blood mononuclear cells is described. The ELISA reproducibly detects as little as one nanogram of IgA in a fifty microliter sample (20 ng/ml).

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