Phenotypic Variation of Salmonella in Host Tissues Delays Eradication by Antimicrobial Chemotherapy
2014; Cell Press; Volume: 158; Issue: 4 Linguagem: Inglês
10.1016/j.cell.2014.06.045
ISSN1097-4172
AutoresBeatrice Claudi, Petra Spröte, Anna Chirkova, Nicolas Personnic, Janine Zankl, Nura Schürmann, Alexander Schmidt, Dirk Bumann,
Tópico(s)Bacterial Genetics and Biotechnology
ResumoSummary Antibiotic therapy often fails to eliminate a fraction of transiently refractory bacteria, causing relapses and chronic infections. Multiple mechanisms can induce such persisters with high antimicrobial tolerance in vitro, but their in vivo relevance remains unclear. Using a fluorescent growth rate reporter, we detected extensive phenotypic variation of Salmonella in host tissues. This included slow-growing subsets as well as well-nourished fast-growing subsets driving disease progression. Monitoring of Salmonella growth and survival during chemotherapy revealed that antibiotic killing correlated with single-cell division rates. Nondividing Salmonella survived best but were rare, limiting their impact. Instead, most survivors originated from abundant moderately growing, partially tolerant Salmonella . These data demonstrate that host tissues diversify pathogen physiology, with major consequences for disease progression and control.
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