ras oncogene activation and the expression of ras-related genes in human lung cancer
1993; Elsevier BV; Volume: 9; Issue: 1-6 Linguagem: Inglês
10.1016/0169-5002(93)90654-g
ISSN1872-8332
AutoresSjoerd Rodenhuis, Peter C. Groot, Gajja S. Salomons, Robert J.C. Slebos,
Tópico(s)PI3K/AKT/mTOR signaling in cancer
ResumoMutational activation of a ras oncogene is detected in about 30% of human adenocarcinomas of the lung. Most mutations involve codon 12 of the K-ras gene. K-ras mutations are rare in non-adenocarcinomas and very rare or non-existent in small-cell lung cancer. K-ras mutations occur with significantly higher frequency in smokers than in non-smokers and indicate a poor prognosis, even in patients with (clinically) early disease after apparently radical operation. It is still uncertain whether the poor prognosis is in part caused by increased resistance to radiation and/or chemotherapy of ras mutation-positive tumors. A possible role of ras-related genes that code for small monomeric GTP binding proteins in human lung cancer is under investigation. The rasac and racA genes were expressed in most, and Krev in all 16 lung cancer samples and 5 lung cancer cell lines investigated. No point mutations were revealed by Single-Strand Conformation Polymorphism (SSCP) analysis in any of these genes, but three samples had unexpected smaller-sized bands on Krev analysis, both under denaturing and non-denaturing conditions. The significance of this finding is being investigated.
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