Visualizing Gold Nanoparticle Uptake in Live Cells with Liquid Scanning Transmission Electron Microscopy
2011; American Chemical Society; Volume: 11; Issue: 4 Linguagem: Inglês
10.1021/nl200285r
ISSN1530-6992
AutoresDiana B. Peckys, Niels de Jonge,
Tópico(s)Advanced biosensing and bioanalysis techniques
ResumoThe intracellular uptake of 30 nm diameter gold nanoparticles (Au-NPs) was studied at the nanoscale in pristine eukaryotic cells. Live COS-7 cells were maintained in a microfluidic chamber and imaged using scanning transmission electron microscopy. A quantitative image analysis showed that Au-NPs bound to the membranes of vesicles, possibly lysosomes, and occupied 67% of the available surface area. The vesicles accumulated to form a micrometer-sized cluster after 24 h of incubation. Two clusters were analyzed and found to consist of 117 ± 9 and 164 ± 4 NP-filled vesicles.
Referência(s)