Synthesis of glucosylceramide by purified calf spleen β-glucosidase
1974; Elsevier BV; Volume: 58; Issue: 1 Linguagem: Inglês
10.1016/0006-291x(74)90896-1
ISSN1090-2104
AutoresSrinivasa S. Raghavan, Richard A. Mumford, Julian N. Kanfer,
Tópico(s)Lysosomal Storage Disorders Research
ResumoRadioactive glucocerebroside was formed when purified calf spleen β-glucosidase was incubated in the presence of 4-methylumbelliferyl-β-D-glucoside and (14C) labeled ceramide. The product was identified by cochromatography on thin layer plates and carrier dilution and crystallization to constant specific activity. The radioactive glucocerebroside was also a substrate for hydrolysis by this same β-glucosidase preparation employed for its synthesis resulting in the liberation of labeled ceramide. Neither methyl-β-D-glucoside nor free D-glucose were effective in producing radioactive glucocerebroside when incubated with labeled ceramide in this system.
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