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The Effect of Surface Active Additives on the Partitioning of Proteins and Enzymes in Aqueous Two-Phase Systems

1996; Wiley; Volume: 67; Issue: 4 Linguagem: Inglês

10.1002/(sici)1097-4660(199612)67

1097-4660

Vilas G. Gaikar, Sangita S. Bodhankar, V. Latha,

thermodynamics and calorimetric analyses

Journal of Chemical Technology & BiotechnologyVolume 67, Issue 4 p. 329-332 Original Paper The effect of surface active additives on the partitioning of proteins and enzymes in aqueous two-phase systems V. G. Gaikar, Corresponding Author V. G. Gaikar Department of Chemical Technology, University of Bombay, Matunga, Mumbai-400019, IndiaDepartment of Chemical Technology, University of Bombay, Matunga, Mumbai-400019, India===Search for more papers by this authorS. S. Bodhankar, S. S. Bodhankar Department of Chemical Technology, University of Bombay, Matunga, Mumbai-400019, IndiaSearch for more papers by this authorV. Latha, V. Latha Department of Chemical Technology, University of Bombay, Matunga, Mumbai-400019, IndiaSearch for more papers by this author V. G. Gaikar, Corresponding Author V. G. Gaikar Department of Chemical Technology, University of Bombay, Matunga, Mumbai-400019, IndiaDepartment of Chemical Technology, University of Bombay, Matunga, Mumbai-400019, India===Search for more papers by this authorS. S. Bodhankar, S. S. Bodhankar Department of Chemical Technology, University of Bombay, Matunga, Mumbai-400019, IndiaSearch for more papers by this authorV. Latha, V. Latha Department of Chemical Technology, University of Bombay, Matunga, Mumbai-400019, IndiaSearch for more papers by this author First published: December 1996 https://doi.org/10.1002/(SICI)1097-4660(199612)67:4 3.0.CO;2-XCitations: 5AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The effect of cationic and anionic surface active additives on the partitioning of proteins has been studied. The surface active agents interact with proteins electrostatically and also hydrophobically. The increased hydrophobicity of the proteins because of association with surfactants is responsible for the changes in the partition coefficients and in some cases for precipitation of the proteins. Specific interactions also exist between mildly surface active amino acids and proteins. REFERENCES 1 Albertsson, P. A., Partition of Cell Particles and Macromolecules, 3rd. edn. Wiley, New York, 1986. Google Scholar 2 Diamond, A. D. & Hsu, J. T., Aqueous two-phase systems for biomolecule separation. Advances in Biomechanical Engineering, 47 (1993) 90–131. Google Scholar 3 Shanbhag, V. P. & Johnsson, G., Specific extraction of human serum albumin by partition in aqueous biphasic systems containing poly(ethylene glycol)-bound ligand. Biochemical and Biophysical Research Communications, 61 (1974) 1141–6. 10.1016/S0006-291X(74)80402-X CASPubMedWeb of Science®Google Scholar 4 Tanford, C., The Hydrophobic Effect. Wiley-Interscience, New York, 1993. Google Scholar 5 Powers, M. E., Matsura, J., Brassell, J., Manning, M. C. & Shefter, E., Enhanced solubility of proteins, peptides in non-polar solvents through hydrophobic ion pairing. Biopolymers, 61 (1993) 927–32. 10.1002/bip.360330608 Web of Science®Google Scholar 6 Agarwal, M. & Gaikar, V. G., Beneficial effect of hydrotropes on the partitioning behaviour of protein in aqueous two-phase systems. Bioseparation, 3 (1993) 233–41. Google Scholar 7 Albertsson, P. A., History of aqueous two-phase systems in Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses and Applications to Biotechnology, eds H. Walter, D. E. Brooks & D. Fisher. Academic Press, Orlando, 1985. Google Scholar 8 Katsumichi, O., Hideo, K. & Kenkichi, M., Conductometric studies of ion-binding to poly(oxyethylene) in methanol. J. Phy. Chem., 83 (1979) 2665–9. 10.1021/j100483a024 Web of Science®Google Scholar Citing Literature Volume67, Issue4December 1996Pages 329-332 ReferencesRelatedInformation