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Wound healing properties and kill kinetics of Clerodendron splendens G. Don, a ghanaian wound healing plant

2010; Medknow; Volume: 2; Issue: 2 Linguagem: Inglês

10.4103/0974-8490.62948

0976-4836

Stephen Yao Gbedema, Kisseih Emelia, Adu Francis, Annan Kofi, Woode Eric,

Wound Healing and Treatments

Pharmacognosy Research,2010,2,2,63-68.DOI:10.4103/0974-8490.62948Published:May 2010Type:Original ArticleAuthors:Stephen Y Gbedema, Kisseih Emelia, Adu Francis, Annan Kofi, and Woode Eric Author(s) affiliations:Stephen Y Gbedema1, Kisseih Emelia1, Adu Francis1, Annan Kofi2, Woode Eric3 1Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, West Africa 2Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, West Africa 3Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, West Africa Abstract:As part of our general objective of investigating indigenous plants used in wound healing in Ghana, we hereby report our findings from some in vitro and in vivo studies related to wound healing activities of Clerodendron splendens G. Don (Verbanaceae). Methanolic extract of the aerial parts of the plant was tested for antimicrobial activity against Gram positive bacteria (Bacillus subtilis, Staphylococcus aureus, Streptococcus faecalis, Micrococcus flavus, as well as resistant strains of Staph. aureus SA1199B, RN4220 and XU212), Gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteous mirabilis, Klebsiella pneumoniae) and Candida albicans using the micro-well dilution method. Survivor-time studies of the microorganisms, radical scavenging activity using 2,2'-diphenylpicrylhydrazyl (DPPH) and various in vivo wound healing activity studies were also conducted on the extract. The extract exhibited biostatic action against all the test microorganisms with a Minimum Inhibition Concentration (MIC) ranging between 64 and 512 μg/ml and a free radical scavenging property with an IC 50 value of 103.2 μg/ml. The results of the in vivo wound healing tests showed that upon application of C. splendens ointment, there was a reduction in the epithelization period from 26.7 days (control) to 13.6 days along with a marked decrease in the scar area from 54.2 mm 2 (control) to 25.2 mm 2 . Significant increase in the tensile strength and hydroxyproline content were also observed as compared to the control and was comparable to nitrofurazone. The above results appear to justify the traditional use of C. splendens in wound healing and treatment of skin infections in Ghana. Keywords:Antimicrobial, Antioxidant, Biostatic, Clerodendron splendens, Wound healingView:PDF (1.05 MB)