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Artigo Produção Nacional Revisado por pares
BIBTEX RIS

Pinealectomy interferes with the circadian clock genes expression in white adipose tissue

2015; Wiley; Volume: 58; Issue: 3 Linguagem: Inglês

10.1111/jpi.12211

ISSN

1600-079X

Autores

Talita da Silva Mendes de Farias, Ariclécio Cunha de Oliveira, Sandra Andreotti, Fernanda Gaspar do Amaral, Patrícia Chimin, André Ricardo Alves de Proença, Francisco Leonardo Torres‐Leal, Rogério Antônio Laurato Sertié, Amanda Baron Campaña, Andressa Bolsoni Lopes, Arnaldo Henrique de Souza, José Cipolla‐Neto, Fábio Bessa Lima,

Tópico(s)

Neurobiology and Insect Physiology Research

Resumo

Melatonin, the main hormone produced by the pineal gland, is secreted in a circadian manner (24-hr period), and its oscillation influences several circadian biological rhythms, such as the regulation of clock genes expression (chronobiotic effect) and the modulation of several endocrine functions in peripheral tissues. Assuming that the circadian synchronization of clock genes can play a role in the regulation of energy metabolism and it is influenced by melatonin, our study was designed to assess possible alterations as a consequence of melatonin absence on the circadian expression of clock genes in the epididymal adipose tissue of male Wistar rats and the possible metabolic repercussions to this tissue. Our data show that pinealectomy indeed has impacts on molecular events: it abolishes the daily pattern of the expression of Clock, Per2, and Cry1 clock genes and Pparγ expression, significantly increases the amplitude of daily expression of Rev-erbα, and affects the pattern of and impairs adipokine production, leading to a decrease in leptin levels. However, regarding some metabolic aspects of adipocyte functions, such as its ability to synthesize triacylglycerols from glucose along 24 hr, was not compromised by pinealectomy, although the daily profile of the lipogenic enzymes expression (ATP-citrate lyase, malic enzyme, fatty acid synthase, and glucose-6-phosphate dehydrogenase) was abolished in pinealectomized animals.

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