Cytosolic Prion Protein Is Not Toxic and Protects against Bax-mediated Cell Death in Human Primary Neurons
2003; Elsevier BV; Volume: 278; Issue: 42 Linguagem: Inglês
10.1074/jbc.m306177200
ISSN1083-351X
AutoresXavier Roucou, Qi Guo, Yan Zhang, Cynthia G. Goodyer, Andréa C. LeBlanc,
Tópico(s)Trace Elements in Health
ResumoRecently, it was observed that reverse-translocated cytosolic PrP and PrP expressed in the cytosol induce rapid death in neurons (Ma, J., Wollmann, R., and Lindquist, S. (2002) Science 298, 1781–1785). In this study, we investigated whether accumulation of prion protein (PrP) in the cytosol is toxic to human neurons in primary culture. We show that in these neurons, a single PrP isoform lacking signal peptide accumulates in the cytosol of neurons treated with epoxomicin, a specific proteasome inhibitor. Therefore, endogenously expressed PrP is subject to the endoplasmic reticulum-associated degradation (ERAD) pathway and is degraded by the proteasome in human primary neurons. In contrast to its toxicity in N2a cells, reverse-translocated PrP (ERAD-PrP) is not toxic even when neurons are microinjected with cDNA constructs to overexpress either wild-type PrP or mutant PrPD178N. We found that ERAD-PrP in human neurons remains detergentsoluble and proteinase K-sensitive, in contrast to its detergent-insoluble and proteinase K-resistant state in N2a cells. Furthermore, not only is microinjection of a cDNA construct expressing CyPrP not toxic, it protects these neurons against Bax-mediated cell death. We conclude that in human neurons, ERAD-PrP is not converted naturally into a form reminiscent of scrapie PrP and that PrP located in the cytosol retains its protective function against Bax. Thus, it is unlikely that simple accumulation of PrP in the cytosol can cause neurodegeneration in prion diseases. Recently, it was observed that reverse-translocated cytosolic PrP and PrP expressed in the cytosol induce rapid death in neurons (Ma, J., Wollmann, R., and Lindquist, S. (2002) Science 298, 1781–1785). In this study, we investigated whether accumulation of prion protein (PrP) in the cytosol is toxic to human neurons in primary culture. We show that in these neurons, a single PrP isoform lacking signal peptide accumulates in the cytosol of neurons treated with epoxomicin, a specific proteasome inhibitor. Therefore, endogenously expressed PrP is subject to the endoplasmic reticulum-associated degradation (ERAD) pathway and is degraded by the proteasome in human primary neurons. In contrast to its toxicity in N2a cells, reverse-translocated PrP (ERAD-PrP) is not toxic even when neurons are microinjected with cDNA constructs to overexpress either wild-type PrP or mutant PrPD178N. We found that ERAD-PrP in human neurons remains detergentsoluble and proteinase K-sensitive, in contrast to its detergent-insoluble and proteinase K-resistant state in N2a cells. Furthermore, not only is microinjection of a cDNA construct expressing CyPrP not toxic, it protects these neurons against Bax-mediated cell death. We conclude that in human neurons, ERAD-PrP is not converted naturally into a form reminiscent of scrapie PrP and that PrP located in the cytosol retains its protective function against Bax. Thus, it is unlikely that simple accumulation of PrP in the cytosol can cause neurodegeneration in prion diseases. The prion protein (PrP) 1The abbreviations used are: PrP, prion protein; ERAD, endoplasmic reticulum-associated degradative pathway; ERAD-PrP, PrP derived from the ERAD and retro-translocated in the cytosol; SP-PrP, PrP containing the N-terminal signal peptide; PrPSc, PrP scrapie; CyPrP, cytosolic form of PrP (residues 23–231); EGFP, enhanced green fluorescent protein; DTR, dextran Texas Red; TUNEL, terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling; BFA, brefeldin A; Tricine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine; ER, endoplasmic reticulum; wtPrP, wild-type PrP. is mostly expressed as a secreted glycoprotein that remains attached to the cell surface through a glycophosphatidylinositol anchor. On occasion, PrP also accumulates as a transmembrane type I or type II glycoprotein (1Yost C.S. Lopez C.D. Prusiner S.B. Myers R.M. Lingappa V.R. Nature. 1990; 343: 669-672Crossref PubMed Scopus (110) Google Scholar). Furthermore, wild-type and mutant PrPs, Y145stop and Q217R, respectively, generate intracellular PrP in the presence of proteasome inhibitors, indicating that the normally secreted PrP is subject to the degradative pathway termed endoplasmic reticulum associated protein degradation (ERAD), possibly to eliminate misfolded PrP molecules (2Zanusso G. Petersen R.B. Jin T. Jing Y. Kanoush R. Ferrari S. Gambetti P. Singh N. J. Biol. Chem. 1999; 274: 23396-23404Abstract Full Text Full Text PDF PubMed Scopus (152) Google Scholar, 3Jin T. Gu Y. Zanusso G. Sy M. Kumar A. Cohen M. Gambetti P. Singh N. J. Biol. Chem. 2000; 275: 38699-38704Abstract Full Text Full Text PDF PubMed Scopus (153) Google Scholar, 4Yedidia Y. Horonchik L. Tzaban S. Yanai A. Taraboulos A. EMBO J. 2001; 20: 5383-5391Crossref PubMed Scopus (223) Google Scholar, 5Ma J. Lindquist S. Proc. Natl. Acad. Sci. U. S. A. 2001; 98: 14955-14960Crossref PubMed Scopus (269) Google Scholar). This form of PrP is termed ERAD-PrP. However, immunodetection of PrP with an intact N-terminal signal peptide in transfected cells treated with a proteasome inhibitor raises the possibility that a fraction of the overexpressed PrP (SP-PrP) is synthesized in the cytosol instead of going through the secretory pathway (6Drisaldi B. Stewart R.S. Adles C. Stewart L.R. Quaglio E. Biasini E. Fioriti L. Chiesa R. Harris D.A. J. Biol. Chem. 2003; 278: 21732-21743Abstract Full Text Full Text PDF PubMed Scopus (196) Google Scholar). The presence of PrP in the cytosol (ERAD-PrP or SP-PrP) suggests that deregulation of the proteasome during aging could result in the accumulation of cytotoxic PrP molecules (7Keller J.N. Gee J. Ding Q. Ageing Res. Rev. 2002; 1: 279-293Crossref PubMed Scopus (209) Google Scholar). Indeed, PrP that accumulates in the cytosol of mouse neuroblastoma N2a cells, Chinese hamster ovary cells, and African green monkey kidney COS-1 cells forms insoluble aggregates partially resistant to proteinase K, which is reminiscent of PrP scrapie (PrPSc) (4Yedidia Y. Horonchik L. Tzaban S. Yanai A. Taraboulos A. EMBO J. 2001; 20: 5383-5391Crossref PubMed Scopus (223) Google Scholar, 5Ma J. Lindquist S. Proc. Natl. Acad. Sci. U. S. A. 2001; 98: 14955-14960Crossref PubMed Scopus (269) Google Scholar, 8Ma J. Lindquist S. Science. 2002; 298: 1785-1788Crossref PubMed Scopus (269) Google Scholar). Furthermore, accumulation of PrP in the presence of proteasome inhibitors is toxic to N2a cells but not to COS-1 or Swiss mouse embryo NIH3T3 fibroblasts, suggesting that cytosolic PrP is toxic only to neuronal cell types (9Ma J. Wollmann R. Lindquist S. Science. 2002; 298: 1781-1785Crossref PubMed Scopus (432) Google Scholar). In vivo, expression of a transgene encoding a form of PrP that lacks both the N- and C-terminal signal peptides and is exclusively expressed in the cytosol (CyPrP) induces a massive loss of granule cerebellar neurons and gliosis (9Ma J. Wollmann R. Lindquist S. Science. 2002; 298: 1781-1785Crossref PubMed Scopus (432) Google Scholar). Because CyPrP is expressed at a very low level in the transgenic model, it is assumed that CyPrP is a very toxic isoform that is responsible for the initiation of neurodegeneration in prion diseases and that it contributes to the production of the protease-resistant PrPSc (9Ma J. Wollmann R. Lindquist S. Science. 2002; 298: 1781-1785Crossref PubMed Scopus (432) Google Scholar). Because of the importance of these findings on the development of prion diseases, we studied the effect of PrP accumulation in the cytosol of human primary neurons. Human neurons are a more appropriate model to address the toxicity of PrP in relation to prion diseases in human, as they may behave differently than cell lines because they are terminally differentiated, polarized, and have a very long life span. We show that in human primary neurons, endogenously expressed PrP accumulates in the cytosol when cells are treated with epoxomicin, a specific irreversible inhibitor of the proteasome. The protein does not possess an N-terminal signal peptide and is, therefore, likely to arise from the ERAD pathway. However, in contrast to N2a cells, ERAD-PrP in the cytosol of human neurons remains proteinase K-sensitive and detergent-soluble and is not toxic. Moreover, overexpression of WtPrP, PrPD178N, or CyPrP from microinjected cDNA constructs is not toxic in human neurons, nor is CyPrP expression toxic in two human neuroblastoma cell lines, BE(2)-M17 and SK-N-SH, treated with epoxomicin. In addition, we demonstrate that CyPrP expression protects human neurons against Bax-mediated cell death, similar to what we previously observed with WtPrP (10Bounhar Y. Zhang Y. Goodyer C.G. LeBlanc A. J. Biol. Chem. 2001; 276: 39145-39149Abstract Full Text Full Text PDF PubMed Scopus (305) Google Scholar). We conclude that accumulation of PrP in the cytosol of neurons is not sufficient for neurotoxicity. Clones—Cloning of human PrP, PrP-D178N, and Bax in pCEP4β (Invitrogen) was described previously (10Bounhar Y. Zhang Y. Goodyer C.G. LeBlanc A. J. Biol. Chem. 2001; 276: 39145-39149Abstract Full Text Full Text PDF PubMed Scopus (305) Google Scholar). CyPrP (residues 23–231) was PCR-amplified using forward primer 5′-ataagaatgcggccgcatgaagaagcgcccgaagcctggag-3′ and reverse primer 5′-cgcggatcctcacatgctcgatcctctctggtaatag-3′ and cloned between the NotI and BamHI sites of pCE-P4β. The pCEP4β-enhanced green fluorescent protein (EGFP)-Bax construct was obtained by PCR amplification of Bax with 5′-ccgctcgagtggacgggtccggggagcagc-3′ and 5′-cccaagctttcagcccatcttcttccacatcc-3′, and the PCR product was cloned in the XhoI/HindIII sites of pCEP4β-EGFP. pCEP4β-EGFP was created by transferring EGFP from EGFP-C1 (Clontech) into the NheI/BamHI restriction sites of pCEP4β. Cell Culture, Microinjections, Transfection, and Cell Death—Human primary neurons were cultured as described (11LeBlanc A. J. Neurosci. 1995; 15: 7837-7846Crossref PubMed Google Scholar). Microinjections and cell death determination were described previously (12Zhang Y. LeBlanc A. Boulton A.A. Baker G.B. Apoptosis Techniques and Protocols. Humana Press, Totowa, NJ2002: 83-106Google Scholar). Briefly, neurons were microinjected with 25 pl containing 0.75 pg of DNA and 2.5 pg of dextran Texas Red (DTR) in phosphate-buffered saline. Two hundred neurons from at least three independent neuronal preparations were injected. Cells were fixed in 4% sucrose and 4% paraformaldehyde in phosphate-buffered saline; cell death was assessed by TUNEL according to the manufacturer's protocol (In Situ Cell Death Detection Kit (A,P), Roche Applied Science). The percentage of cell death was determined as the number of DTR and TUNEL double-positive over the total number of DTR-positive neurons. All cells were obtained from American Type Culture Collection (Manassas, VA). Human neuroblastoma cells BE(2)-M17 and SK-N-SH were maintained in OptiMEM plus 10% fetal bovine serum and minimum essential medium plus 5% bovine calf serum, respectively (Invitrogen). N2a mouse neuroblastoma cells were maintained in Dulbecco's modified Eagle's medium plus 10% fetal bovine serum (Invitrogen). Transfections were carried out in 6-well plates using ExGen 500 and 4 μg of DNA according to the manufacturer's protocol (MBI, Burlington, Ontario, Canada). For detection of SP-PrP, BE(2)-M17 cells were transfected with 10 μg of DNA, as described previously (6Drisaldi B. Stewart R.S. Adles C. Stewart L.R. Quaglio E. Biasini E. Fioriti L. Chiesa R. Harris D.A. J. Biol. Chem. 2003; 278: 21732-21743Abstract Full Text Full Text PDF PubMed Scopus (196) Google Scholar). Nuclei were visualized after staining 20 min with 1 μg/ml Hoechst 33342 (Sigma) 24 h after transfection. Subcellular Fractionation and PrP Detection—Subcellular fractions were prepared as described previously (13Desagher S. Osen-Sand A. Nichols A. Eskes R. Montessuit S. Lauper S. Maundrell K. Antonsson B. Martinou J.C. J. Cell Biol. 1999; 144: 891-901Crossref PubMed Scopus (1093) Google Scholar) with some modification. Approximately 72 × 106 cells were washed three times with ice-cold phosphate-buffered saline buffer and homogenized with 20 strokes in a Potter-Elvehjem homogenizer (homogenization buffer: 8% sucrose (w/v), 20 mm HCl-Tricine, pH 7.8, 1 mm EDTA). After a first spin at 2000 × g for 10 min to eliminate unbroken cells and nuclei, the supernatant was centrifuged at 100,000 × g for 30 min. Supernatant and pellet from the 100,000 × g centrifugation represent the cytosolic and membrane fractions, respectively. Proteins of each fraction were solubilized in loading buffer (0.5% SDS (w/v), 1.25% 2-β-mercaptoethanol (v/v), 4% glycerol (v/v), 0.01% bromphenol blue (w/v), 15 mm Tris-HCl, pH 6.8), and 100 μg of protein was separated in a 15% SDS-PAGE. PrP was detected by Western blotting using the 3F4 monoclonal antibody (1: 5000 dilution) and an antibody directed against PrP N-terminal signal peptide (anti SP-PrP, 1:1000 dilution, kindly provided by Dr. D. A. Harris, Washington University School of Medicine). For Bcl2 and tau detection, a polyclonal N19 (Santa Cruz Biotechnology Inc., Santa Cruz, CA) and a monoclonal T-5530 antibody (Sigma) were used, respectively. Analysis of PrP Aggregation and Proteinase K Digestion—Culture media were replaced with media containing 5 μg/ml brefeldin A (Sigma) and either 0.25 μm (cell lines) or 1 μm (neurons) epoxomicin (BioMol, Plymouth Meeting, PA) 24 h after transfection. After 16 h of treatment, cells in 6-well plates were lysed with 0.2 ml of lysis buffer (50 mm Tris-HCl, pH 7.5, 150 mm NaCl, 2 mm EDTA, 0.5% Triton X-100 (v/v), and 0.5% sodium deoxycholate (w/v)) for 20 min at 4 °C. After centrifugation at 13,000 rpm for 10 min at 4 °C, soluble PrP was present in the supernatant and aggregated PrP was present in the pellet. Proteins from the pellet were resuspended in 0.2 ml of lysis buffer. Proteins in both supernatants and pellets were precipitated with 4 volumes of methanol and analyzed by Western blotting. Resistance to proteinase K was assessed by incubating cell lysates with 10 μg/ml proteinase K for 30 min at 37 °C. Statistical Evaluation—The significance of variance was analyzed with analysis of variance followed by post-hoc Scheffé's test using StatView (SAS Institute Inc., Cary, NC). A p < 0.05 was taken as a significant difference. To assess whether PrP is subject to the ERAD pathway in human neurons, we treated cells with both brefeldin A (BFA) and epoxomicin. BFA blocks the transport of secreted proteins beyond the endoplasmic reticulum (ER)-cis-Golgi compartment (14Misumi Y. Miki K. Takatsuki A. Tamura G. Ikehara Y. J. Biol. Chem. 1986; 261: 11398-11403Abstract Full Text PDF PubMed Google Scholar) and is expected to increase the amount of PrP diverted to the ERAD. Epoxomicin is a specific and irreversible inhibitor of the proteasome (15Meng L. Mohan R. Kwok B.H. Elofsson M. Sin N. Crews C.M. Proc. Natl. Acad. Sci. U. S. A. 1999; 96: 10403-10408Crossref PubMed Scopus (827) Google Scholar) and is expected to allow PrP accumulation in the cytosol. Cells were homogenized, and the presence of PrP was analyzed by Western blotting of proteins from crude extracts, cytosol, and membrane subcellular fractions (Fig. 1A). In untreated cells, PrP was mainly detected in membranes but not in the cytosol. Treatment of cells with BFA + epoxomicin resulted in the synthesis of immature PrP. Moreover, a large proportion of PrP was present in the cytosol. The presence of Bcl2 in membranes but not in the cytosol and the detection of tau protein isoforms mainly in the cytosol show that relatively pure subcellular fractions were used in this experiment. The apparent molecular mass of PrP in the cytosol was slightly decreased (Fig. 1A). To determine if the signal peptide was removed from PrP, we used an antibody directed against the N-terminal signal peptide of PrP (6Drisaldi B. Stewart R.S. Adles C. Stewart L.R. Quaglio E. Biasini E. Fioriti L. Chiesa R. Harris D.A. J. Biol. Chem. 2003; 278: 21732-21743Abstract Full Text Full Text PDF PubMed Scopus (196) Google Scholar) (Fig. 1A). The SP-PrP antibody did not detect PrP in the cytosol, suggesting the form of PrP that accumulated in the presence of epoxomicin and BFA was indeed reverse-translocated. Interestingly, no SP-PrP was detected in the crude lysate of neurons. Therefore, either the signal peptide was efficiently removed from endogenously expressed PrP protein, or the amount of SP-PrP was below the limit of detection. The specificity of the SP-PrP antibody was verified in BE(2)-M17 cells transfected with 10 μg of WtPrP cDNA (Fig. 1B), as described previously (6Drisaldi B. Stewart R.S. Adles C. Stewart L.R. Quaglio E. Biasini E. Fioriti L. Chiesa R. Harris D.A. J. Biol. Chem. 2003; 278: 21732-21743Abstract Full Text Full Text PDF PubMed Scopus (196) Google Scholar). The antibody detects a form of PrP that contains the SP in WtPrP-transfected BE(2)-M17 cells. In contrast, neither fractionated cytosolic PrP nor PrP in crude extracts from control or BFA + epoxomycin-treated human neurons were detected with the SP-antibody. The expression of PrP was verified by reprobing the blot with the 3F4 antibody (Fig. 1B). To determine whether the presence of ERAD-PrP in the cytosol of human neurons is as toxic as it is in neuroblastoma N2a cells (9Ma J. Wollmann R. Lindquist S. Science. 2002; 298: 1781-1785Crossref PubMed Scopus (432) Google Scholar), cell death in untreated or BFA + epoxomicin-treated neurons was assessed by TUNEL assay. Accumulation of PrP in the cytosol does not induce cell death (Fig. 1C, Ctl). To test whether toxicity may occur only in conditions of overexpression of PrP, neurons were microinjected with a WtPrP cDNA high expression construct before the incubation with BFA + epoxomicin. Again, no toxicity was observed (Fig. 1C). Furthermore, microinjection of a construct expressing mutant D178NPrP known to enhance PrP trafficking through the ERAD pathway (5Ma J. Lindquist S. Proc. Natl. Acad. Sci. U. S. A. 2001; 98: 14955-14960Crossref PubMed Scopus (269) Google Scholar) also does not induce toxicity. However, neurons remain sensitive to Bax overexpression in the absence or presence of BFA + epoxomicin. To address more precisely the cytotoxicity of PrP in the cytosol of human neurons, a cytosolic form of PrP (CyPrP, amino acids 23–231) that contains neither the N-terminal ER-targeting peptide nor the C-terminal signal peptide required for the addition of a glycophosphatidylinositol anchor was cloned. This construct expressed stable levels of CyPrP in neuroblastoma BE(2)-M17 cells that lack endogenous PrP expression (Fig. 1D, inset). Microinjection of CyPrP cDNA did not induce neuronal cell death even 72 h after injection (Fig. 1D). In contrast, N2a cells are susceptible to CyPrP expression within 24 h of transfection, as shown by Ma, Wollmann, and Lindquist (9Ma J. Wollmann R. Lindquist S. Science. 2002; 298: 1781-1785Crossref PubMed Scopus (432) Google Scholar) (Fig. 1E). Moreover, attempts to select stable N2a cells expressing CyPrP failed (not shown). This finding prompted us to test the cytotoxicity of CyPrP in other human neuroblastoma cell lines. In contrast to what we observed in N2a murine neuroblastoma cells, expression of CyPrP was toxic to neither BE(2)-M17 nor SK-N-SH cells (Fig. 1E). These cells are all competent to undergo apoptosis, as verified by overexpression of Bax. Therefore, not all neuronal cells are susceptible to CyPrP-mediated toxicity. When it accumulates in the cytosol of N2a cells, ERAD-PrP/SP-PrP is converted to a detergent-insoluble and proteinase K-resistant form reminiscent of the transmissible PrPSc (8Ma J. Lindquist S. Science. 2002; 298: 1785-1788Crossref PubMed Scopus (269) Google Scholar). Therefore, we tested the detergent solubility and proteinase K resistance of PrP in primary neurons or transiently transfected N2a, BE(2)-M17, or SK-N-SH cell lines in the absence and presence of BFA + epoxomicin (Fig. 2). In the absence of BFA + epoxomicin, PrP is completely detergent soluble in all cell types. However, in the presence of BFA + epoxomicin, a small amount of PrP resisted detergent solubility in N2a cells but not in primary neurons and BE(2)-M17 or SK-N-SH cells (Fig. 2A). Similarly, PrP became partially resistant to proteinase K in N2a but not in primary neurons and BE(2)-M17 or SK-N-SH cells treated with BFA + epoxomicin (Fig. 2B). These results show that the conversion of ERAD-PrP does not occur significantly in human primary neurons or neuroblastoma cell lines. To assess whether CyPrP retains its neuroprotective function against Bax in human neurons, we co-microinjected a Bax-expressing construct with CyPrP. Microinjection of Bax cDNA killed about 45% of neurons within 24 h (Fig. 3A). As observed previously, co-expression of WtPrP completely protects against Bax-mediated cell death. Co-injection of CyPrP cDNA also protects against Bax-mediated cell death. To ensure that WtPrP and CyPrP do not inhibit Bax expression, an EGFP-Bax fusion protein cDNA previously shown to behave like WtBax (16Wolter K.G. Hsu Y.T. Smith C.L. Nechushtan A. Xi X.G. Youle R.J. J. Cell Biol. 1997; 139: 1281-1292Crossref PubMed Scopus (1578) Google Scholar) was cloned and microinjected with WtPrP or CyPrP constructs. In the absence of WtPrP and CyPrP co-injection, EGFP was detected in only 25 of the 100 cells injected, indicating that the fusion Bax-EGFP protein may be very toxic and that dying neurons either degrade EGFP-Bax more efficiently or have a lower synthesis rate (Fig. 3B). However, in the presence of WtPrP or CyPrP, 70–80 of 100 cells injected showed EGFP fluorescence, indicating that WtPrP or CyPrP do not inhibit Bax expression but inhibit Bax-mediated cell death. Therefore, not only is expression of PrP in the cytosol not toxic in human neurons, but it also protects against Bax-mediated cytotoxicity. In this paper, we show that endogenously expressed PrP is subject to the ERAD pathway in human primary neurons and that ERAD-PrP does not convert into a PrPSc-like state in the presence of a proteasome inhibitor. In the cytosol, neither ERAD-PrP nor transfected CyPrP is toxic. Moreover, CyPrP inhibits Bax-mediated cell death. PrP Goes through the ERAD Pathway in Human Neurons— Here, we show conclusively that endogenously expressed PrP goes through the ERAD pathway in primary human neurons. Previous studies used immunofluorescence in transfected Chinese hamster ovary, COS, N2a, NIH3T3, and undifferentiated rat pheochromocytoma PC12 cell lines to detect PrP in the cytosol of cells treated with proteasome inhibitors (4Yedidia Y. Horonchik L. Tzaban S. Yanai A. Taraboulos A. EMBO J. 2001; 20: 5383-5391Crossref PubMed Scopus (223) Google Scholar, 5Ma J. Lindquist S. Proc. Natl. Acad. Sci. U. S. A. 2001; 98: 14955-14960Crossref PubMed Scopus (269) Google Scholar, 6Drisaldi B. Stewart R.S. Adles C. Stewart L.R. Quaglio E. Biasini E. Fioriti L. Chiesa R. Harris D.A. J. Biol. Chem. 2003; 278: 21732-21743Abstract Full Text Full Text PDF PubMed Scopus (196) Google Scholar). However, immunofluorescence experiments cannot exclude the possibility that PrP is associated with cytoplasmic structures or organelles. Our observation (obtained by subcellular fractionation) that PrP is detected in the cytosol strongly supports the presence of soluble cytosolic PrP in human neurons. Moreover, expression of PrP under the control of a strong promoter such as cytomegalovirus might artificially produce PrP molecules bearing the N-terminal signal peptide (SP-PrP) that accumulate in the presence of proteasome inhibitors (6Drisaldi B. Stewart R.S. Adles C. Stewart L.R. Quaglio E. Biasini E. Fioriti L. Chiesa R. Harris D.A. J. Biol. Chem. 2003; 278: 21732-21743Abstract Full Text Full Text PDF PubMed Scopus (196) Google Scholar). In human neurons, ERAD-PrP is made from endogenously expressed PrP, and the N-terminal signal peptide is removed from the protein because an antibody directed against this peptide does not detect PrP in the cytosol of cells treated with epoxomicin. ERAD-PrP is detected in a purified cytosolic fraction but not in a crude extract, indicating that the protein is present at a relatively low level. The shift in molecular weight of ERAD-PrP implies that the molecule has lost its immature glycosylation, as expected in a retro-translocated protein. These results indicate that prior to proteasomal degradation, PrP is first translocated into the ER where the signal peptides are cleaved and immature glycosylation occurs. Then, after undergoing deglycosylation, PrP can be reverse-translocated into the cytosol, leading to the appearance of ERAD-PrP. Conversion of Cytosolically Located PrP into a PrPSc-like Isoform Is Limited to Certain Neuronal Cell Types—When ERAD-PrP/SP-PrP accumulates in the cytosol of N2a cells, it converts into a form reminiscent of PrPSc and is toxic (Ref. 8Ma J. Lindquist S. Science. 2002; 298: 1785-1788Crossref PubMed Scopus (269) Google Scholar and this study). Therefore, it was important to determine the state and the toxicity of ERAD-PrP in human neurons. Here, we show evidence that in human primary neurons and in two human neuroblastoma cell lines, BE(2)-M17 and SK-N-SH, ERAD-PrP does not convert into a PrPSc-like form. Several hypotheses could explain why ERAD-PrP conversion seems to be cell type-dependent. First, overexpression of PrP in transfected cells may induce PrP aggregation and resistance to proteinase K. Overexpression-induced protein aggregation in transfected cells has already been reported for α-synuclein (involved in Parkinson's disease) (18McLean P.J. Kawamata H. Hyman B.T. Neuroscience. 2001; 104: 901-912Crossref PubMed Scopus (190) Google Scholar) and tau (involved in tauopathies in transfected cells) (17Hall G.F. Lee V.M. Lee G. Yao J. Am. J. Pathol. 2001; 158: 235-246Abstract Full Text Full Text PDF PubMed Scopus (55) Google Scholar). As mentioned by Drisaldi et al. (6Drisaldi B. Stewart R.S. Adles C. Stewart L.R. Quaglio E. Biasini E. Fioriti L. Chiesa R. Harris D.A. J. Biol. Chem. 2003; 278: 21732-21743Abstract Full Text Full Text PDF PubMed Scopus (196) Google Scholar), proteasome inhibitors may cause a significant increase in PrP expression from the cytomegalovirus promoter, leading to accumulation of large amounts of PrP in cells. The hypothesis that PrP overexpression leads to PrP aggregation in human neurons is supported because they only express endogenous levels of PrP and there is no PrP conversion. However, SK-N-SH cells consistently accumulate more ERAD-PrP than N2a cells, yet they do not produce detergent-insoluble and proteinase K-resistant PrP (Fig. 2A). Second, intracellular redox conditions could affect conversion of PrP into a PrPSc-like form. We have not tested this hypothesis in human neurons and N2a cells, but evidence obtained in yeast and mammalian cells treated with a reducing agent show that cellular PrP is converted into a detergent-insoluble and proteinase K-resistant form (9Ma J. Wollmann R. Lindquist S. Science. 2002; 298: 1781-1785Crossref PubMed Scopus (432) Google Scholar, 19Ma J. Lindquist S. Nat. Cell Biol. 1999; 1: 358-361Crossref PubMed Scopus (105) Google Scholar). Therefore, it is plausible that the reducing conditions in the cytosol vary in different cell types and affect conversion of PrP. Interestingly, recombinant PrP under reducing conditions also forms insoluble aggregates in vitro (20Maiti N.R. Surewicz W.K. J. Biol. Chem. 2001; 276: 2427-2431Abstract Full Text Full Text PDF PubMed Scopus (104) Google Scholar). Third, other cellular parameters such as pH may be involved in PrP conversion. Acidic pH favors PrP conversion in vitro, and some in vivo studies suggest that PrP conversion occurs in intracellular acidic compartments (21Zou W.Q. Cashman N.R. J. Biol. Chem. 2002; 277: 43942-43947Abstract Full Text Full Text PDF PubMed Scopus (80) Google Scholar, 22Borchelt D.R. Taraboulos A. Prusiner S.B. J. Biol. Chem. 1992; 267: 16188-16199Abstract Full Text PDF PubMed Google Scholar, 23Caughey B. Raymond G.J. Ernst D. Race R.E. J. Virol. 1991; 65: 6597-6603Crossref PubMed Google Scholar, 24Swietnicki W. Petersen R. Gambetti P. Surewicz W.K. J. Biol. Chem. 1997; 272: 27517-27520Abstract Full Text Full Text PDF PubMed Scopus (242) Google Scholar). Interestingly, human neurons maintain stable pH, as evidenced by phenol red pH indicator in culture medium, whereas the pH in the culture medium of N2a cells becomes acidic rapidly. Finally, because ERAD-PrP does not convert into a scrapie-like conformation in all cells, it is possible that some cytosolic factors allow or prevent PrP conversion. Chaperones GroEL, a close homolog of mammalian Hsp60, and Hsp104 promote the conversion of PrP into PrPSc-like protein in vitro (25DebBurman S.K. Raymond G.J. Caughey B. Lindquist S. Proc. Natl. Acad. Sci. U. S. A. 1997; 94: 13938-13943Crossref PubMed Scopus (229) Google Scholar, 26Edenhofer F. Rieger R. Famulok M. Wendler W. Weiss S. Winnacker E.L. J. Virol. 1996; 70: 4724-4728Crossref PubMed Google Scholar, 27Stockel J. Hartl F.U. J. Mol. Biol. 2001; 313: 861-872Crossref PubMed Scopus (51) Google Scholar). Cytosolically Located PrP Is Not Toxic in All Neuronal Cell Types—Our results show that ERAD-PrP is not toxic to human primary neurons even when WtPrP or mutant PrPD178N, known to increase trafficking through the ERAD pathway, are overexpressed (5Ma J. Lindquist S. Proc. Natl. Acad. Sci. U. S. A. 2001; 98: 14955-14960Crossref PubMed Scopus (269) Google Scholar). Similar to ERAD-PrP, CyPrP is clearly not toxic in human primary neurons or in human neuroblastoma BE(2)-M17 and SK-N-SH cells. Based on the observation that CyPrP is toxic to N2a cells but not to fibroblast-derived NIH3T3 cells, it was suggested that CyPrP toxicity is restricted to neuronal cells (9Ma J. Wollmann R. Lindquist S. Science. 2002; 298: 1781-1785Crossref PubMed Scopus (432) Google Scholar). Our results show that CyPrP toxicity is, in fact, restricted to few neuronal cells. This conclusion is consistent with the lack of CyPrP toxicity in neuronal cells other than cerebellar granule neurons in transgenic mice, despite the use of the ubiquitously expressed PrP promoter (9Ma J. Wollmann R. Lindquist S. Science. 2002; 298: 1781-1785Crossref PubMed Scopus (432) Google Scholar). It is tempting to speculate that ERAD-PrP toxicity in N2a cells depends on its conversion into a detergent-insoluble and proteinase K-resistant form. To address this question, agents that can prevent ERAD-PrP conversion in N2a cells will need to be uncovered and tested. However, the toxicity of detergent-soluble and proteinase K-sensitive CyPrP in N2a but not NIH3T3 cells and the absence of detectable PrPSc-like CyPrP in the dying cerebellar granule neurons of the transgenic mice argue against this mechanism (9Ma J. Wollmann R. Lindquist S. Science. 2002; 298: 1781-1785Crossref PubMed Scopus (432) Google Scholar). To understand fully why CyPrP or ERAD-PrP is toxic to N2a but not to human neurons or human neuroblastoma cell lines, the underlying mechanism of cell death will have to be elucidated in N2a cells. CyPrP Inhibits Bax-mediated Cell Death—In the present work, we observed that CyPrP is capable of preventing Bax from killing human primary neurons, as we have shown previously with WtPrP (10Bounhar Y. Zhang Y. Goodyer C.G. LeBlanc A. J. Biol. Chem. 2001; 276: 39145-39149Abstract Full Text Full Text PDF PubMed Scopus (305) Google Scholar). Bax has been shown to be critical in several in vitro and in vivo models of neuronal cell death (28Deckwerth T.L. Elliott J.L. Knudson C.M. Johnson Jr., E.M. Snider W.D. Korsmeyer S.J. Neuron. 1996; 17: 401-411Abstract Full Text Full Text PDF PubMed Scopus (657) Google Scholar, 29Harris C.A. Johnson Jr., E.M. J. Biol. Chem. 2001; 276: 37754-37760Abstract Full Text Full Text PDF PubMed Google Scholar, 30Lei K. Nimnual A. Zong W.X. Kennedy N.J. Flavell R.A. Thompson C.B. Bar-Sagi D. Davis R.J. Mol. Cell. Biol. 2002; 22: 4929-4942Crossref PubMed Scopus (443) Google Scholar). Bax is also a very efficient killer of human primary neurons (10Bounhar Y. 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However, the observations that WtPrP goes through the ERAD pathway (2Zanusso G. Petersen R.B. Jin T. Jing Y. Kanoush R. Ferrari S. Gambetti P. Singh N. J. Biol. Chem. 1999; 274: 23396-23404Abstract Full Text Full Text PDF PubMed Scopus (152) Google Scholar, 3Jin T. Gu Y. Zanusso G. Sy M. Kumar A. Cohen M. Gambetti P. Singh N. J. Biol. Chem. 2000; 275: 38699-38704Abstract Full Text Full Text PDF PubMed Scopus (153) Google Scholar, 4Yedidia Y. Horonchik L. Tzaban S. Yanai A. Taraboulos A. EMBO J. 2001; 20: 5383-5391Crossref PubMed Scopus (223) Google Scholar, 5Ma J. Lindquist S. Proc. Natl. Acad. Sci. U. S. A. 2001; 98: 14955-14960Crossref PubMed Scopus (269) Google Scholar), that both WtPrP and CyPrP inhibit Bax killing (Ref. 10Bounhar Y. Zhang Y. Goodyer C.G. LeBlanc A. J. Biol. Chem. 2001; 276: 39145-39149Abstract Full Text Full Text PDF PubMed Scopus (305) Google Scholar and this study), and that four identical N-terminal PrP octapeptide repeats share similarity with the Bcl2 homology domain 2 (39LeBlanc A. Wang S.S. Handbook of the Aging Brain. Academic Press, NY1998: 201-221Crossref Google Scholar) raise the possibility that, in a manner similar to Bcl2, PrP may interact directly with Bax. However, Bax and PrP did not interact in a yeast two-hybrid system experiment (40Kurschner C. Morgan J.I. Brain Res. Mol. Brain Res. 1995; 30: 165-168Crossref PubMed Scopus (150) Google Scholar). Moreover, PrP is present mainly at the cell surface and in luminal vesicular sites (ER, Golgi, endosomes), whereas only a small proportion is retro-translocated (10% of nascent PrP molecules in a cell culture model) (4Yedidia Y. Horonchik L. Tzaban S. Yanai A. Taraboulos A. EMBO J. 2001; 20: 5383-5391Crossref PubMed Scopus (223) Google Scholar). Further studies are required to understand how PrP prevents Bax-mediated cell death. In summary, we have demonstrated that not only is CyPrP not toxic in human primary neurons, it also protects these cells against the major neuronal proapoptotic protein, Bax. Therefore, based on the observation that expression of CyPrP in transgenic mice leads to neurodegeneration, it seems premature to conclude that reverse-translocation of PrP in the cytosol represents a step in the development of prion diseases in humans. This study also highlights the importance of using, in addition to cell lines and animal models, cultured human primary neurons as a cell model of prion diseases. We thank Dr. D. A. Harris (Washington University School of Medicine, St. Louis) for providing the antibodies directed against the N-terminal signal peptide of PrP.
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