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2006 GEP-ISFG collaborative exercise on mtDNA: reflections about interpretation, artefacts, and DNA mixtures

2007; Elsevier BV; Volume: 2; Issue: 2 Linguagem: Inglês

10.1016/j.fsigen.2007.10.010

1878-0326

Lourdes Prieto, Antonio Alonso, Cı́ntia Alves, Manuel Crespillo, M. Montesino, A. Picornell, António Brehm, José Ramírez, M.R. Whittle, M.J. Anjos, Ilaria Boschi, J. Buj, María Cerezo, Sergio Cardoso, Regina Maria Barretto Cicarelli, David Comas, Daniel Corach, Christian Doutremépuich, R. Espinheira, I Fernández-Fernández, S. Filippini, Julia Garcı́a-Hirschfeld, Alberto González, B. Heinrichs, A. Hernández, Fabio P.N. Leite, R.P. Lizarazo, Ana Marı́a López-Parra, Manolo López-Soto, José A. Lorente, Búrix Mechoso, Isabela Cunha Navarro, S. Pagano, J. Pestano, Jorge Puente, Eduardo Raimondi, A. Rodríguez-Quesada, M.F. Terra-Pinheiro, Lidia Vidal-Rioja, Carlos Vullo, Antonio Salas,

Identification and Quantification in Food

We report the results of the seventh edition of the GEP-ISFG mitochondrial DNA (mtDNA) collaborative exercise. The samples submitted to the participant laboratories were blood stains from a maternity case and simulated forensic samples, including a case of mixture. The success rate for the blood stains was moderate ( approximately 77%); even though four inexperienced laboratories concentrated about one-third of the total errors. A similar success was obtained for the analysis of mixed samples (78.8% for a hair-saliva mixture and 69.2% for a saliva-saliva mixture). Two laboratories also dissected the haplotypes contributing to the saliva-saliva mixture. Most of the errors were due to reading problems and misinterpretation of electropherograms, demonstrating once more that the lack of a solid devised experimental approach is the main cause of error in mtDNA testing.