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Regeneration of the Vocal Fold Using Autologous Mesenchymal Stem Cells

2003; SAGE Publishing; Volume: 112; Issue: 11 Linguagem: Inglês

10.1177/000348940311201101

1943-572X

Shin‐ichi Kanemaru, Hisayoshi Kojima, Shigeru Hirano, Tatsuo Nakamura, Akhmar Magrufov, Juichi Ito, Koichi Omori, Yasuyuki Hiratsuka, Yasuhiko Shimizu,

Tissue Engineering and Regenerative Medicine

The aim of this study was to regenerate the injured vocal fold by means of selective cultured autologous mesenchymal stem cells (MSCs). Eight adult beagle dogs were used for this experiment. Selective incubation of MSCs from bone marrow was done. These MSCs were submitted to 3-dimensional incubation in 1% hydrochloric acid atelocollagen. Three-dimensional incubated MSCs were injected into the left vocal fold, and atelocollagen only was injected into the right vocal fold of the same dog as a control. Four days after injection, the posterior parts of the vocal folds were incised. The regeneration of the vocal fold was estimated by morphological and histologic evaluations. Our results showed that 3-dimensional incubated MSCs were useful in the regeneration of the injured vocal fold. This study shows that damaged tissues such as an injured vocal fold would be able to be regenerated by tissue engineering.