Thermitase – eine thermostabile Serinprotease. II. Synthese von substratanalogen Peptidchlormethylketonen als potentielle irreversible Enzyminhibitoren
1984; Wiley; Volume: 326; Issue: 1 Linguagem: Inglês
10.1002/prac.19843260107
ISSN0021-8383
AutoresGünther Jahreis, K. Smalla, S. Fittkau,
Tópico(s)Biochemical and Structural Characterization
ResumoThermitase – A Thermostable Serine Protease. II. Synthesis of Substrate Analogous Peptide Chloromethyl Ketones as Irreversibly Acting Inhibitors The preparation of dipeptide to pentapeptide chloromethyl ketones of alanine wth variation of the amino acid in the P 1 and P 2 position Nomenklatur für die individuellen Aminosäurereste der Peptide nach I. Schechter u. A. C. Berger, Biochem. Biophys. Res. Commun. 27 , 157 (1967). of the peptides is described. The synthesis is performed by fragment condensation of N‐acylated amino acids or peptides with the unprotected chloromethyl ketone derivatives of amino acids and peptides, respectively. Some examples of enzyme inactivation by peptide chloromethyl ketones are discussed.
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