Two Radish Genes for 3-Hydroxy-3-Methylglutaryl-CoA Reductase Isozymes Complement Mevalonate Auxotrophy in a Yeast Mutant and Yield Membrane-Bound Active Enzyme
1994; Elsevier BV; Volume: 143; Issue: 4-5 Linguagem: Inglês
10.1016/s0176-1617(11)81810-9
ISSN1618-1328
AutoresKai‐Uwe Vollack, Bärbel Dittrich, Albert Ferrer, Albert Boronat, Thomas J. Bach,
Tópico(s)Biofuel production and bioconversion
ResumoExpression of two full-length cDNAs encoding two related isozymes of radish (Raphanus sativus L. var. Saxa Knacker) 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR, EC 1.1.1.34) can complement mevalonate auxotrophy in a yeast mutant having both HMGR genes (HMG1, HMG2) disrupted [see Basson et al., 1988]. HMGR activity in transformants, placed under the control of the GAL10 promoter, can be induced in the presence of galactose and is exclusively localized to membranes. Yeast HMGRs contain seven or more membrane-spanning hydrophobic regions, but functional complementation and evidently targeting to yeast membranes shows that the two membrane spans present in plant HMGRs are already sufficient for membrane insertion.
Referência(s)