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Major Proteomic Changes Associated with Amyloid-Induced Biofilm Formation in Pseudomonas aeruginosa PAO1

2014; American Chemical Society; Volume: 14; Issue: 1 Linguagem: Inglês

10.1021/pr500938x

1535-3907

Florian‐Alexander Herbst, Mads T. Søndergaard, Henrik Kjeldal, Allan Stensballe, Per Halkjær Nielsen, Morten Simonsen Dueholm,

Oral microbiology and periodontitis research

The newly identified functional amyloids in Pseudomonas (Fap) are associated with increased aggregation and biofilm formation in the opportunistic pathogen P. aeruginosa; however, whether this phenomenon can be simply ascribed to the mechanical properties of the amyloid fibrils remains undetermined. To gain a deeper understanding of the Fap-mediated biofilm formation, the physiological consequences of Fap expression were investigated using label-free protein quantification. The functional amyloids were found to not solely act as inert structural biofilm components. Their presence induced major changes in the global proteome of the bacterium. These included the lowered abundance of classical virulence factors such as elastase B and the secretion system of alkaline protease A. Amyloid-mediated biofilm formation furthermore increased abundance of the alginate and pyoverdine synthesis machinery, which turned P. aeruginosa PAO1 into an unexpected mucoid phenotype. The results imply a significant impact of functional amyloids on the physiology of P. aeruginosa with subsequent implications for biofilm formation and chronic infections.