Medullary carcinoma of thyroid gland. Subcellular distribution of calcitonin and relationship between granules and amyloid
1973; Wiley; Volume: 31; Issue: 2 Linguagem: Inglês
10.1002/1097-0142(197302)31
ISSN1097-0142
AutoresJohn S. Meyer, William E. Hutton, Alexander D. Kenny,
Tópico(s)Protein Hydrolysis and Bioactive Peptides
ResumoElectron microscopy and bioassay for calcitonin were used to study two intact medullary carcinomas and their subcellular fractions. The fractions were prepared by sucrose gradient ultracentrifugation after removal of mitochondria. Virtually all of the calcitonin was bound to subcellular particles, but it was distributed among all of the particulate fractions that were obtained, and consequently it cannot be assigned to any one kind of particle. The two types of particles with which calcitonin most probably is associated are secretory granules and microsomes. Secretory granules also were closely related to the amyloid of the medullary carcinoma, and we suggest that the granules play a role in formation of the amyloid.
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