Inhibition of HLA B8-restricted recognition by unrelated peptides: evidence for allosteric inhibition
1991; Elsevier BV; Volume: 30; Issue: 3 Linguagem: Inglês
10.1016/0165-2478(91)90048-f
ISSN1879-0542
AutoresAnnette Fernan, Scott R. Burrows, Denis J. Moss, Allan Saul, Andreas Suhrbier,
Tópico(s)Immune Cell Function and Interaction
ResumoA panel of synthetic peptides representing human lymphocyte antigen (HLA) B8, other class I and class II restricted T cell epitopes and two B cell epitopes, were all able to compete with recognition of a HLA B8 restricted epitope by a cytotoxic T cell clone. Competition was obtained when the competitor peptides were added either before or after the target epitope. The target epitope also had a slow off rate, implicating allosteric inhibition. The presence of non-specific, allosteric binding sites may interfere with experiments attempting to define immunologically relevant MHC binding specificities.
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