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BIBTEX RIS

Purification and Characterization of Three MEKA-Like Proteins in Liver: Association of a 94 kDa Protein with β γ Subunits of G-Proteins

1993; Elsevier BV; Volume: 191; Issue: 3 Linguagem: Inglês

10.1006/bbrc.1993.1329

ISSN

1090-2104

Autores

Che‐Hui Kuo, Eiichi Taira, Natsuki Takaha, Hitoshi Sohma, T Akino, Yoshitaka Fukada, Kamon Sanada, N Miki,

Tópico(s)

RNA regulation and disease

Resumo

Retinal 32 kDa MEKA protein (rMEKA) exists in the photoreceptor cells and forms a complex with β γ subunit of transducin. Bovine liver contained three MEKA-like proteins (94 kDa, 35 kDa-a, 35 kDa-b) which reacted with a rMEKA antibody. Each protein was purified as a single band on a SDS-PAGE and used for a reconstitution experiment with α and β γ subunits of cerebral G-proteins (Go/i). The 94 kDa protein inhibited GTP-binding ability of Gα by forming a complex with β γ subunit.

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