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Multiplex detection of Enterocytozoon bieneusi and Encephalitozoon spp. in fecal samples using real-time PCR

2006; Elsevier BV; Volume: 57; Issue: 2 Linguagem: Inglês

10.1016/j.diagmicrobio.2006.08.009

1879-0070

Jaco J. Verweij, Robert-Jan ten Hove, Eric A. T. Brienen, Lisette van Lieshout,

Amoebic Infections and Treatments

A multiplex real-time polymerase chain reaction (PCR) method was developed for the simultaneous detection of Enterocytozoon bieneusi (n = 30) and Encephalitozoon spp. (n = 3) in stool samples. The multiplex PCR also included an internal control to detect inhibition of the amplification by fecal constituents in the sample. The assay was performed on species-specific DNA controls (n = 22) and a range of well-defined stool samples (n = 140), and it achieved 100% specificity and sensitivity. The use of this assay in a diagnostic laboratory offers the possibility of introducing DNA detection as a feasible technique in the routine diagnosis of intestinal microsporidian infections.