Cyclodextrin−Surfactant Complex: A New Route in DNA Decompaction
2008; American Chemical Society; Volume: 9; Issue: 3 Linguagem: Inglês
10.1021/bm7012907
ISSN1526-4602
AutoresAlfredo González‐Pérez, Rita S. Dias, Tommy Nylander, Björn Lindman,
Tópico(s)Drug Solubulity and Delivery Systems
ResumoIn the present work, we show a new approach for decompaction of DNA−cationic surfactant complexes, e.g., lipoplexes, by using β-cyclodextrin (β-CD). The DNA decompaction was achieved by dissolving the surfactant aggregates in the complex by making use of the high affinity between the β-CD and the free surfactant in solution. The results from fluorescence microscopy and adiabatic compressibility measurements indicate that coils and globules do not coexist. The reported procedure using β-CD is an efficient way to decompact DNA surfactant complexes because the association constant of surfactants with β-CD is large. The surfactant's interaction with β-CD is specific and the nonspecific interaction between β-CD and biological interfaces is small.
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