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Oligonucleotide-based targeted gene editing in C. elegans via the CRISPR/Cas9 system

2014; Springer Nature; Volume: 24; Issue: 2 Linguagem: Inglês

10.1038/cr.2014.9

1748-7838

Pei Zhao, Zhe Zhang, Hongmei Ke, Yiren Yue, Ding Xue,

Innovation and Socioeconomic Development

Technologies to achieve specific and precise genome editing, such as knock-in and knock-out, are critical for deciphering the functions of a gene and for understanding fundamental biological processes. Compared with the zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN), which have been used for genome editing1, the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system has emerged as a new powerful tool for genome modifications. It has recently been adopted for genome editing in human cell lines2,3,4, mouse5, zebrafish6, C. elegans7,8,9,10,11,12, and plants13.