Transformation of Bacillus thuringiensis vegetative cells by electroporation
1989; Oxford University Press; Volume: 60; Issue: 3 Linguagem: Inglês
10.1111/j.1574-6968.1989.tb03485.x
ISSN1574-6968
AutoresLuke Masson, Gabrielle Préfontaine, Roland Brousseau,
Tópico(s)Bacillus and Francisella bacterial research
ResumoA highly efficient procedure for the transformation of Bacillus thuringiensis and Bacillus subtilis using covalently closed circular plasmid DNA was developed by using the small Staphylococcus aureus plasmid pC194 and electroporation. We have achieved transformation efficiencies in B. thuringiensis subsp. kurstaki (HD-73) greater than 5 × 106 transformants/µg plasmid DNA. The electro-transformation (or electroporation) procedure also worked with B. subtilis 168 although at a 200-fold less level of efficiency. The results indicated that the plasmid exists in double and single-stranded forms both in B. subtilis and B. thuringiensis. A second single-stranded species was also observed in both species. This technique may prove to be applicable to other members of the genus Bacillus.
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