Evidence that Xenopus laevis contains two different nonallelic insulin-like growth factor-I genes
1990; Elsevier BV; Volume: 166; Issue: 1 Linguagem: Inglês
10.1016/0006-291x(90)91934-k
ISSN1090-2104
AutoresAlan R. Shuldiner, Ajay Nirula, Laurie Scott, Jesse Roth,
Tópico(s)Thyroid Disorders and Treatments
ResumoUsing the polymerase chain reaction (PCR), we have amplified and characterized partial nucleotide sequences of two distinct insulin-like growth factor-I genes (designated IGF-I′ and IGF-I″) from the amphibian, Xenopuslaevis. The amplified fragments encoded much of the coding region of the mature peptide (exon III in mammalian IGF-I genes), and exhibited 93% similarity to each other, and 68–82% similarity to mammalian IGF-I amino acid sequences. Southern blot analysis using genomic DNA from a homozygous frog revealed that these two genes are nonallelic in a single organism, like the two nonallelic genes encoding Xenopus insulins that we have characterized previously. Furthermore, both IGF-I mRNAs are expressed in similar quantities in adult liver.
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