Produção Nacional
Revisado por pares
Desenvolvimento e validação de método analítico para determinação simultânea de lamivudina, zidovudina e nevirapina em comprimidos dose-fixa combinada por cromatografia líquida de alta eficiência
2008; Brazilian Chemical Society; Volume: 31; Issue: 5 Linguagem: Inglês
10.1590/s0100-40422008000500005
ISSN1678-7064
AutoresZênia Maria Maciel Lavra, Pedró José Rolim Neto, Rosali Maria Ferreira da Silva, Flávia Patrícia Morais de Medeiros,
Tópico(s)HIV/AIDS drug development and treatment
ResumoLIQUIDCHROMATOGRAPHY.An analytical method has been developed and validated for the quantitation of lamivudine, zidovudine and nevirapine in the fixed-dose combination film-coated tablet by high performance liquid chromatography, in accordance with RE No. 899/2003, National Sanitary Surveillance Agency.It was based on an isocratic elution system with a potassium phosphate buffer pH 3.0: acetonitrile (60:40 v/v) mobile phase, C18, 250 x 46 mm column, 10μm particle size, λ 270 nm.The statistically evaluated results have shown that the method is specific, precise, accurate, and robust, ensuring the analytical safety of 3TC, AZT and NVP determination, which emerges as a new therapeutic alternative for antiretroviral treatment.
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