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Improved protein resolution of concentrated tissue extract in zonal column electrophoresis

1960; Elsevier BV; Volume: 38; Linguagem: Inglês

10.1016/0006-3002(60)91295-6

1878-2434

S Sorof, Emily M. Young, Mike Spence, Phyllis L. Fetterman,

Genetics, Bioinformatics, and Biomedical Research

This chapter discusses electromigration techniques. Paper electrophoresis represents one of the most early developed electromigration techniques. Apparatuses used for paper electrophoresis is composed of of two electrode jars and a system that holds the paper sheet between them and prevents, at least partly, the imbedded buffer from evaporation. The equipment used could be categorized into two groups with either horizontal or vertical arrangement of the paper strip. Electrophoresis in gel media represents the first application of an electrophoretic procedure in combination with a sieving effect. The apparatus for this purpose is very simple. It consists of two buffer vessels with platinum electrodes and a gel tray placed between them. The contact of the gel layer with electrode buffers is ensured by wicks of thick chromatographic paper. The gel tray is composed of a plastic base (about 10 × 15 cm) and two identical frames placed on top of each other. The whole system is held together by rubber bands. There are several commercially available instruments of this type (e.g., Desaga Disaphor, Savant Model HGE 312) all provided with a cooling system. The chapter also discusses gradient gel electrophoresis, two-dimensional polyacrylamide gel electrophoresis and the Isodalt system, agarose gel electrophoresis, and others.