Portal de Periódicos da CAPES

All-solid-state lock-in imaging for wide-field fluorescence lifetime sensing

2005; Optica Publishing Group; Volume: 13; Issue: 24 Linguagem: Inglês

10.1364/opex.13.009812

1094-4087

Alessandro Esposito, Thierry Oggier, Hans C. Gerritsen, Felix Lustenberger, Fred S. Wouters,

Photoacoustic and Ultrasonic Imaging

Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful technique that is increasingly being used in the life sciences during the past decades. However, a broader application of FLIM requires more cost-effective and user-friendly solutions. We demonstrate the use of a simple CCD/CMOS lock-in imager for fluorescence lifetime detection. The SwissRanger SR-2 time-of-flight detector, originally developed for 3D vision, embeds all the functionalities required for FLIM in a compact system. The further development of this technology and its combination with light-emitting-and laser diodes could drive a wider spreading of thuse of FLIM including high-throughput applications.