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Production, Purification, and Properties of an Extracellular Laccase from Rigidoporus lignosus

2000; Elsevier BV; Volume: 18; Issue: 2 Linguagem: Inglês

10.1006/prep.1999.1126

1096-0279

Maria Teresa Cambria, Antonio Cambria, Santa Ragusa, Enrico Rizzarelli,

melanin and skin pigmentation

Laccase from Rigidoporus lignosus, a white-rot basidiomycete, has been isolated from culture filtrates. The enzyme was purified to homogeneity and some of its structural and kinetic parameters have been determined. The effects of pH, temperature, and organic solvents on the activity and stability of the enzyme, under different conditions, were also assayed. The results we have obtained, including the rather broad substrate specificity of enzyme, combined with their relatively easy production and purification, suggest that laccase may be efficiently employed in a variety of biotechnology applications.