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Successive detection of insulin-like growth factor-II bound to receptors on a living cell surface using an AFM

2009; Wiley; Volume: 24; Issue: 1 Linguagem: Inglês

10.1002/jmr.994

1099-1352

Sung-Woong Han, Shingo Mieda, Chikashi Nakamura, Takanori Kihara, Noriyuki Nakamura, Jun Miyake,

Microfluidic and Bio-sensing Technologies

Journal of Molecular RecognitionVolume 24, Issue 1 p. 17-22 Research Article Successive detection of insulin-like growth factor-II bound to receptors on a living cell surface using an AFM† Sung-Woong Han, Sung-Woong Han Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, JapanSearch for more papers by this authorShingo Mieda, Shingo Mieda Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, JapanSearch for more papers by this authorChikashi Nakamura, Corresponding Author Chikashi Nakamura [email protected] Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, JapanResearch Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.Search for more papers by this authorTakanori Kihara, Takanori Kihara The Center for NanoBio Integration, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, JapanSearch for more papers by this authorNoriyuki Nakamura, Noriyuki Nakamura Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, JapanSearch for more papers by this authorJun Miyake, Jun Miyake Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan The Center for NanoBio Integration, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, JapanSearch for more papers by this author Sung-Woong Han, Sung-Woong Han Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, JapanSearch for more papers by this authorShingo Mieda, Shingo Mieda Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, JapanSearch for more papers by this authorChikashi Nakamura, Corresponding Author Chikashi Nakamura [email protected] Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, JapanResearch Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan.Search for more papers by this authorTakanori Kihara, Takanori Kihara The Center for NanoBio Integration, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, JapanSearch for more papers by this authorNoriyuki Nakamura, Noriyuki Nakamura Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, JapanSearch for more papers by this authorJun Miyake, Jun Miyake Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan The Center for NanoBio Integration, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, JapanSearch for more papers by this author First published: 30 December 2010 https://doi.org/10.1002/jmr.994Citations: 10 † This paper was presented at AFM BioMed Monterey 2008. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract In this study, we have developed a method of mechanical force detection for ligands bound to receptors on a cell surface, both of which are involved in a signal transduction pathway. This pathway is an autocrine pathway, involving the production of insulin-like growth factor-II (IGF-II) and activation of the IGF-I receptor, involved in myoblast differentiation induced by MyoD in C3H10T1/2 mouse mesenchymal stem cells. Differentiation of C3H10T1/2 was induced with the DNA demethylation agent 5-azacytidine (5-aza). The etched AFM tip used in the force detection had a flat surface of which about 10 µm2 was in contact with a cell surface. The forces required to rupture the interactions of IGF-IIs on a cell and anti mouse IGF-II polyclonal antibody immobilized on an etched AFM tip were measured within 5 days of induction of differentiation. The mean unbinding force for a single paired antibody–ligand on a cell was about 81 pN, which was measured at a force loading rate of about 440 nN/s. The percentage of unbinding forces over 100 pN increased to 32% after 2 days from the addition of 5-aza to the medium. This method could be used in non-invasive and successive evaluation of a living cell's behavior. Copyright © 2009 John Wiley & Sons, Ltd. REFERENCES Benoit DS, Schwartz MP, Durney AR, Anseth KS. 2008. 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