A study of human coagulation factor XIII A-subunit by electrospray ionisation mass spectrometry
2000; Wiley; Volume: 14; Issue: 17 Linguagem: Inglês
10.1002/1097-0231(20000915)14
ISSN1097-0231
AutoresAlison E. Ashcroft, Peter J. Grant, Robert A. S. Ari�ns,
Tópico(s)Erythropoietin and Anemia Treatment
ResumoRapid Communications in Mass SpectrometryVolume 14, Issue 17 p. 1607-1611 Research Article A study of human coagulation factor XIII A-subunit by electrospray ionisation mass spectrometry Alison E. Ashcroft, Corresponding Author Alison E. Ashcroft a.e.ashcroft@leeds.ac.uk Astbury Centre for Structural Molecular Biology, School of Biochemistry and Molecular Biology, The University of Leeds, Leeds LS2 9JT, UKAstbury Centre for Structural Molecular Biology, School of Biochemistry and Molecular Biology, The University of Leeds, Leeds LS2 9JT, UKSearch for more papers by this authorPeter J. Grant, Peter J. Grant Unit of Molecular Vascular Medicine, School of Medicine, The University of Leeds, Leeds LS2 9JT, UKSearch for more papers by this authorRobert A. S. Ariëns, Robert A. S. Ariëns Unit of Molecular Vascular Medicine, School of Medicine, The University of Leeds, Leeds LS2 9JT, UKSearch for more papers by this author Alison E. Ashcroft, Corresponding Author Alison E. Ashcroft a.e.ashcroft@leeds.ac.uk Astbury Centre for Structural Molecular Biology, School of Biochemistry and Molecular Biology, The University of Leeds, Leeds LS2 9JT, UKAstbury Centre for Structural Molecular Biology, School of Biochemistry and Molecular Biology, The University of Leeds, Leeds LS2 9JT, UKSearch for more papers by this authorPeter J. Grant, Peter J. Grant Unit of Molecular Vascular Medicine, School of Medicine, The University of Leeds, Leeds LS2 9JT, UKSearch for more papers by this authorRobert A. S. Ariëns, Robert A. S. Ariëns Unit of Molecular Vascular Medicine, School of Medicine, The University of Leeds, Leeds LS2 9JT, UKSearch for more papers by this author First published: 23 August 2000 https://doi.org/10.1002/1097-0231(20000915)14:17 3.0.CO;2-LCitations: 14Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract A study of factor XIII A-subunit has been made by electrospray ionisation mass spectrometry. Factor XIII has been isolated and rigorously purified, and the intact A-subunit protein has been analysed to confirm its molecular weight accurately for the first time. Thrombin proteolysis of the A-subunit has been monitored and the resulting cleavage products, the activated A-subunit protein and the Activation Peptide, have been analysed by electrospray ionisation mass spectrometry to confirm the site of proteolysis. Facile and rapid separation of the Activation Peptide from the activated A-subunit protein has been achieved using simple on-line separation techniques. The molecular weight measurements have been repeated numerous times to provide an indication of the precision and variability of the analyses. Copyright © 2000 John Wiley & Sons, Ltd. Citing Literature Volume14, Issue1715 September 2000Pages 1607-1611 RelatedInformation
Referência(s)