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Simultaneous determination of amphetamines and ketamines in urine by gas chromatography/mass spectrometry

2006; Wiley; Volume: 20; Issue: 11 Linguagem: Inglês

10.1002/rcm.2499

1097-0231

Huei Ru Lin, Ahai C. Lua,

Neurotransmitter Receptor Influence on Behavior

Abstract A method for the simultaneous determination of amphetamines and ketamines (ketamine, norketamine and dehydronorketamine) in urine samples by gas chromatography/mass spectrometry was developed and validated. Urine samples were extracted with organic solvent and derivatized with trifluoroacetic anhydride (TFAA). The limits of detection and limits of quantification for each analyte were lower than 19 and 30 ng/mL, respectively. Within‐day and between‐day precisions were within 0.5% and 10.6%, respectively. Biases for three levels of control samples were within −10.6% and +7.8%. The concentration of dehydronorketamine was greater than those of ketamine or norketamine in 19 of 35 ketamine‐positive samples. A group of 110 human urine samples previously determined to contain at least one of the target analytes was analyzed using the new method, and excellent agreement was observed with previous results. Copyright © 2006 John Wiley & Sons, Ltd.