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Free amino acid profiles of aerobic (red) and anaerobic (white) skeletal muscle of the cyprinid fish, Carassius auratus L. (goldfish)

1989; Elsevier BV; Volume: 94; Issue: 4 Linguagem: Inglês

10.1016/0300-9629(89)90639-7

0300-9629

Jeroen van der Boon, Guido E.E.J.M. van den Thillart, Albert D.F. Addink,

Aldose Reductase and Taurine

1. A reversed-phase high performance liquid chromatographic technique (with o-phthaldialdehyde derivatization and UV-detection) was employed to compare the free amino acid profiles of aerobic and anaerobic skeletal muscle types of fed, normoxic goldfish. 2. Both muscle types differ significantly in levels of free histidine, taurine, glycine, valine, isoleucine, glutamic acid and aspartic acid and are very rich in the nonprotein amino acid derivative, 2-aminoethane-sulfonic acid (taurine), and the essential free amino acid, histidine. 3. Goldfish red muscle free amino acid pool consists of 50% taurine, 25% histidine and 25% other free amino acids, while in the white muscle the distribution is 40% taurine, 30% histidine and 30% other free amino acids.