Portal de Periódicos da CAPES

Photocurrent generation properties of Histag-photosystem II immobilized on nanostructured gold electrode

2007; Elsevier BV; Volume: 516; Issue: 9 Linguagem: Inglês

10.1016/j.tsf.2007.04.127

1879-2731

Nao Terasaki, Masako Iwai, Noritaka Yamamoto, Takashi Hiraga, Sunao Yamada, Yasunori Inoue,

Photoreceptor and optogenetics research

It is well known that photosystem II (PSII) can produce electrons, oxygen, and protons simultaneously via a water-splitting photoreaction. These photochemical properties are expected to exhibit photoconductive function for PSII. In the present study, we have first observed a stable photocurrent due to the photoexcitation of PSII and the subsequent water-splitting reaction, by successfully immobilizing PSII on the self-assembled monolayer (SAM) of a nickel-nitrilotriacetic acid complex (Ni-NTA) prepared on a gold surface via a polyhistidine tag (Histag) as a linker molecule. We have further succeeded in the fabrication of PSII-gold nanoparticle multistructures on the surface of gold electrode, and significant enhancement of photocurrents was achieved due to increased number of immobilized PSII.