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A non-curved chicken lysozyme 5' matrix attachment site is 3' followed by a strongly curved DNA sequence

1990; Oxford University Press; Volume: 18; Issue: 13 Linguagem: Inglês

10.1093/nar/18.13.3881

ISSN

1362-4962

Autores

Jens Peter von Kries, Loc Phi‐van, Stephen Diekmann, Wolf H. Strätling,

Tópico(s)

Bacterial Genetics and Biotechnology

Resumo

Matrix attachment regions (MARs) partition the genome into functional and structural loop-domains. Here, we determined the relative matrix affinity of cloned fragments of the chicken lysozyme 5' MAR. We show that this region contains a non-curved high-affinity binding site, which is 3' followed by a strongly curved DNA sequence that exhibits weak matrix binding. DNA curvature is not a physical property required for strong matrix binding. Possible biological functions of this sequence arrangement, particularly of the strongly curved DNA, are discussed.

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