Cellular localization of herpes simplex virus by means of fluorescent antibody
1956; Elsevier BV; Volume: 2; Issue: 4 Linguagem: Inglês
10.1016/0042-6822(56)90006-x
ISSN1096-0341
Autores Tópico(s)Viral Infectious Diseases and Gene Expression in Insects
ResumoThe cellular localization of the antigens of herpes simplex virus was studied by means of specific fluorescent antibody in a race of human epidermoid carcinoma cells growing in tissue culture. Observations made at various intervals during the development of the viral infection were correlated with the appearance of the cells stained with hematoxylin and eosin, and the relation of the antigenic material to the developing inclusion body was determined. Viral antigen first made its appearance as a tiny round spot well inside the nucleus, followed by the development of increasing numbers of such spherical areas and the gradual appearance of a network of antigenic material inside the nucleus, until the nucleus contained a large homogenous area of viral antigen. This large area was basophilic, however, and did not correspond to the typical type A intranuclear inclusion. Specific antigenic material appeared diffusely in the cytoplasm of the infected cell, increasing in amount, whereas the antigenic material in the nucleus decreased until it became small or absent. Cells in this end stage of the process showed a typical type A intranuclear inclusion; this inclusion therefore contains little or no virus, representing an "intranuclear scar." These findings confirm those of other workers using the Feulgen reaction and the electron microscope. Incidental observations were also made on the infected chorioallantoic membrane of the developing chick embryo and on the intracerebrally infected mouse brain.
Referência(s)