Transferred NOE analyses of conformations of peptides as bound to membrane bilayer of phospholipid; mastoparan‐X
1983; Wiley; Volume: 162; Issue: 1 Linguagem: Inglês
10.1016/0014-5793(83)81062-x
ISSN1873-3468
AutoresKaori Wakamatsu, Tsutomu Higashijima, Masahiko Fujino, Terumi Nakajima, Tatsuo Miyazawa,
Tópico(s)Lipid Membrane Structure and Behavior
ResumoThe 270‐MHz 1 H‐NMR spectra of mastoparan‐X, a tetradecapeptide toxin from hornet venom, have been observed in the presence of vesicles of perdeuterated dilauroylphosphatidylcholine ([ 2 H 64 ]DLPC). By the analysis of transferred nuclear Overhauser effects (TRNOE), the mastoparan‐X molecule as bound to [ 2 H 64 ]DLPC vesicles is found to take an α‐helical form in the C‐terminal (and central) part. The TRNOE analysis with perdeuterated phospholipid is a unique method for the elucidation of conformation of peptide (and drug) molecules as bound to phospholipid membranes.
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