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Three‐dimensional multiphoton/optical coherence tomography for diagnostic applications in dermatology

2012; Wiley; Volume: 6; Issue: 4 Linguagem: Inglês

10.1002/jbio.201200085

1864-0648

Aneesh Alex, Jessika Weingast, Martin Weinigel, Marcel Kellner‐Höfer, Romina Nemecek, Michael Binder, Hubert Pehamberger, Karsten König, Wolfgang Drexler,

Photoacoustic and Ultrasonic Imaging

Abstract A preliminary clinical trial using state‐of‐the‐art multiphoton tomography (MPT) and optical coherence tomography (OCT) for three‐dimensional (3D) multimodal in vivo imaging of normal skin, nevi, scars and pathologic skin lesions has been conducted. MPT enabled visualization of sub‐cellular details with axial and transverse resolutions of <2 μm and <0.5 μm, respectively, from a volume of 0.35 × 0.35 × 0.2 mm 3 at a frame rate of 0.14 Hz (512 × 512 pixels). State‐of‐the‐art OCT, operating at a center wavelength of 1300 nm, was capable of acquiring 3D images depicting the layered architecture of skin with axial and transverse resolutions ∼8 μm and ∼20 μm, respectively, from a volume of 7 × 3.5 × 1.5 mm 3 at a frame rate of 46 Hz (1024 × 1024 pixels). This study demonstrates the clinical diagnostic potential of MPT/OCT for pre‐screening relatively large areas of skin using 3D OCT to identify suspicious regions at microscopic level and subsequently using high resolution MPT to obtain zoomed in, sub‐cellular level information of the respective regions (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)