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Potassium-39 nuclear magnetic resonance

1970; Elsevier BV; Volume: 40; Issue: 5 Linguagem: Inglês

10.1016/0006-291x(70)90916-2

1090-2104

Robert G. Bryant,

Electron Spin Resonance Studies

Potassium nuclear magnetic resonance is investigated as a tool for studying potassium ion interactions in biochemistry. The potassium line width in 2 M potassium chloride is 7.1 ± 0.6 Hz. The line is substantially broadened in the presence of inorganic phosphate and adenosine triphosphate at pH values greater than 6. It is shown that the potassium-39 line is broadened significantly by the addition of pyruvate kinase thus demonstrating a direct interaction of the metal ion with the enzyme in the absence of added cofactors.