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Raman spectroscopic study of triplex-like complexes of polyuridylic acid with the isopolar, non-isosteric phosphonate analogues of diadenosine monophosphate

1999; Wiley; Volume: 30; Issue: 8 Linguagem: Inglês

10.1002/(sici)1097-4555(199908)30

1097-4555

Jaroslav Hanuš, Klára Chmelová, Josef Štěpánek, P. Y. Turpin, J. Bok, Ivan Rosenberg, Z. Točík,

DNA and Nucleic Acid Chemistry

Journal of Raman SpectroscopyVolume 30, Issue 8 p. 667-676 Research Article Raman spectroscopic study of triplex-like complexes of polyuridylic acid with the isopolar, non-isosteric phosphonate analogues of diadenosine monophosphate J. Hanuš, J. Hanuš Charles University, Institute of Physics, Ke Karlovu 5, CZ-121 16 Prague 2, Czech Republic Université Pierre et Marie Curie, LPBC (CNRS URA 2056), 4 Place Jussieu, Case 138, F-75252 Paris Cedex 05, FranceSearch for more papers by this authorK. Chmelová, K. Chmelová Charles University, Institute of Physics, Ke Karlovu 5, CZ-121 16 Prague 2, Czech Republic Université Pierre et Marie Curie, LPBC (CNRS URA 2056), 4 Place Jussieu, Case 138, F-75252 Paris Cedex 05, FranceSearch for more papers by this authorJ. Štěpánek, Corresponding Author J. Štěpánek [email protected] Charles University, Institute of Physics, Ke Karlovu 5, CZ-121 16 Prague 2, Czech RepublicCharles University, Institute of Physics, Ke Karlovu 5, CZ-121 16 Prague 2, Czech RepublicSearch for more papers by this authorP.-Y. Turpin, P.-Y. Turpin Université Pierre et Marie Curie, LPBC (CNRS URA 2056), 4 Place Jussieu, Case 138, F-75252 Paris Cedex 05, FranceSearch for more papers by this authorJ. Bok, J. Bok Charles University, Institute of Physics, Ke Karlovu 5, CZ-121 16 Prague 2, Czech RepublicSearch for more papers by this authorI. Rosenberg, I. Rosenberg Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, CZ-166 10 Prague 6, Czech RepublicSearch for more papers by this authorZ. Točík, Z. Točík Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, CZ-166 10 Prague 6, Czech RepublicSearch for more papers by this author J. Hanuš, J. Hanuš Charles University, Institute of Physics, Ke Karlovu 5, CZ-121 16 Prague 2, Czech Republic Université Pierre et Marie Curie, LPBC (CNRS URA 2056), 4 Place Jussieu, Case 138, F-75252 Paris Cedex 05, FranceSearch for more papers by this authorK. Chmelová, K. Chmelová Charles University, Institute of Physics, Ke Karlovu 5, CZ-121 16 Prague 2, Czech Republic Université Pierre et Marie Curie, LPBC (CNRS URA 2056), 4 Place Jussieu, Case 138, F-75252 Paris Cedex 05, FranceSearch for more papers by this authorJ. Štěpánek, Corresponding Author J. Štěpánek [email protected] Charles University, Institute of Physics, Ke Karlovu 5, CZ-121 16 Prague 2, Czech RepublicCharles University, Institute of Physics, Ke Karlovu 5, CZ-121 16 Prague 2, Czech RepublicSearch for more papers by this authorP.-Y. Turpin, P.-Y. Turpin Université Pierre et Marie Curie, LPBC (CNRS URA 2056), 4 Place Jussieu, Case 138, F-75252 Paris Cedex 05, FranceSearch for more papers by this authorJ. Bok, J. Bok Charles University, Institute of Physics, Ke Karlovu 5, CZ-121 16 Prague 2, Czech RepublicSearch for more papers by this authorI. Rosenberg, I. Rosenberg Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, CZ-166 10 Prague 6, Czech RepublicSearch for more papers by this authorZ. Točík, Z. Točík Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, CZ-166 10 Prague 6, Czech RepublicSearch for more papers by this author First published: 02 August 1999 https://doi.org/10.1002/(SICI)1097-4555(199908)30:8 3.0.CO;2-5Citations: 18AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract The formation of poly(rU) complexes with diadenosine monophosphate (3′–5′) and its four isopolar, non-isosteric phosphonate analogues was studied by means of Raman spectroscopic titration. A set of mixed samples containing the diadenosine monophosphate or monophosphonate and the poly(rU) at various concentration ratios was prepared. The room temperature spectra of mixed solutions were treated by factor analysis and decomposition of the Raman marker bands. For all the species studied, the results revealed an exclusive presence of the triplex-like complexes, i.e. complexes with a 1:2 stoichiometric ratio of the adenosine to uridine units, the Raman spectra of which exhibit all the features typical of the poly(rU)·poly(rA)·poly(rU) triple-helical structure. The individual molar fractions of the free ApA dimer, the free poly(rU) and the triplex-like complex do not fit classical equilibrium equation, because the binding ability of the adenosine dimers to poly(rU) falls significantly for small ApA molar fractions. 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