Studies of antigen-antibody interaction on some specific solid adsorbents derived from cellulose
1972; Elsevier BV; Volume: 9; Issue: 10 Linguagem: Inglês
10.1016/0019-2791(72)90108-5
ISSN1878-237X
AutoresNorman Weliky, Howard H. Weetall,
Tópico(s)Biopolymer Synthesis and Applications
ResumoStudies of the dissociation of complexes using specific insoluble complexing agents under specified conditions, can yield information concerning the nature of the specific interactions contributing to complex formation. For the case of the interaction of specific antibody with antigen conjugates, data is obtained concerning heterogeneity of apparent equilibrium constants and the influence of antigen and immunoadsorbent structure on protein interaction and antigen-antibody dissociation. Such information can be useful for predicting conditions for antiserum purification or antibody recovery. For the case of the cellulose adsorbents chosen, it was found that (1) the adsorbent may contribute strongly to the interaction of ionizable haptens with antibody, with a pH dependency typical of acid catalyzed reactions (2) immunoadsorbent properties can be modified through physical and chemical structural changes (3) the dissociation of antibody from the haptenic and protein immunoadsorbents showed no significant discontinuities in the acid range and (4) antibodies to those haptens chosen which do not ionize in the acid range, do not dissociate appreciably from the immunoadsorbent in the acid range but do at sufficiently high pH.
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