Portal de Periódicos da CAPES

Antigenicity of p ‐Azobenzenearsonate‐Containing Oligopeptides in Rabbits

1974; Wiley; Volume: 44; Issue: 1 Linguagem: Inglês

10.1111/j.1432-1033.1974.tb03482.x

1432-1033

Howard A. Levin, Martin Becker, Michael Sela,

Immune Response and Inflammation

The denned compounds containing p ‐azobenzenearsonate (Aba), Aba‐Tyr, Aba‐Tyr‐Tyr, Aba‐Tyr‐(Tyr) 2 , Aba‐Tyr‐(Tyr) 3 , (Tyr) 3 ‐Aba‐Tyr and Aba‐Tyr‐(Ala) 3 (where Tyr is l ‐tyrosine and Ala is l ‐alanine) were synthesized and were investigated immunologically in New Zealand white rabbits. After immunization with the peptides in complete Freund's adjuvant the antibody responses were monitored by both a hapten‐modified bacteriophage inactivation assay and quantitative antibody precipitation. The anti‐Aba antibody response was shown to increase as tyrosine residues were added to the Aba‐Tyr moiety, with the most significant jump in titer occurring between Aba‐Tyr‐Tyr and Aba‐Tyr‐(Tyr) 2 . In contradistinction, no increase in immunogenicity was observed when three alanyl residues were used to lengthen the peptide chain. Studies of the specificity of the anti‐Aba antibodies produced against the peptides, using inhibition of the hapten‐modified phage inactivation, indicated that, while the specificity of antibodies is direct mainly to the Aba‐Tyr moiety in these compounds, the antibodies did demonstrate specificity for the homologous immunizing antigen. The data suggests that the area adjacent to the Aba‐Tyr group on these small immunogens is important for its recognition as an immunogen and also for its ability to determine the specificity of the antibody induced.