Mapping the SRY gene in Microtus cabrerae : a vole species with multiple SRY copies in males and females
2002; NRC Research Press; Volume: 45; Issue: 3 Linguagem: Inglês
10.1139/g02-007
ISSN1480-3321
AutoresRosa Fernández, María José López Barragán, Mónica Bullejos, Juan Alberto Marchal, Sérgio Martínez, Rafael Díaz de la Guardia, Antonio Sánchez,
Tópico(s)Genomics and Chromatin Dynamics
ResumoThe SRY gene is a single-copy, male-specific gene, located on the Y chromosome in most mammals. However, recently we have described the presence of multiple polymorphic copies of this gene in both males and females of the vole species Microtus cabrerae. Here, we present the chromosomal localization of SRY gene copies in this species by fluorescent in situ hybridization (FISH). This technique localized these gene copies in the short arm, and hence in the euchromatic region, of the Y chromosome. Furthermore, several copies of the SRY gene are located on the X chromosome. These copies are spread along the entire heterochromatic region of the X chromosome, occupying the whole short arm, the centromeric region, and the pericentromeric region of the long arm.Key words: FISH mapping, Micotus cabrerae, SRY gene, X chromosome, Y chromosome.
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