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Isolation techniques for HeLa-cell nuclei

1962; Elsevier BV; Volume: 65; Issue: 3 Linguagem: Inglês

10.1016/0006-3002(62)90452-3

1878-2434

P. Srinivasan, A. Miller‐Faurès, M. Errera,

RNA and protein synthesis mechanisms

Three methods of isolation of nuclei of HeLa cells were investigated in connection with studies of pulse-labeled RNA in HeLa cells: (a) sucrose-homogenization, (b) osmotic shock, (c)_citric acid. Of the three methods only the nuclei isolated by citric acid method conserved all the radioactivity of the pulse-labeled RNA found in the nuclei of whole cells. Isolation by the other two methods resulted in a 20 and 17% loss of the labeled RNA of the nuclei.