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Crystallization of fragment D from human fibrinogen

1995; Wiley; Volume: 4; Issue: 5 Linguagem: Inglês

10.1002/pro.5560040523

1469-896X

Stephen J. Everse, H. Pelletier, Russell F. Doolittle,

Enzyme Structure and Function

Abstract Fragment D from human fibrinogen has been crystallized. The fragment, which is composed of three disulfide‐linked chains (α' β' γ' = 88,000), was generated with either plasmin or mild trypsin digestion. The crystals diffracted out to 3.5 Å; the space group is P2 1 , unit cell dimensions a = 108 Å, b = 48 Å, c = 167 Å, β = 106°. Fragment D was also co‐crystallized with the ligand GPRP‐amide, in which case the space group is consistent with P 2 1 2 1 2 1 , unit cell dimensions a = 476 Å, b = 82 Å, c = 432 Å.