Expression of selenoprotein-coding genes SEPP1, SEP15 and hGPX1 in non-small cell lung cancer
2008; Elsevier BV; Volume: 65; Issue: 1 Linguagem: Inglês
10.1016/j.lungcan.2008.10.023
ISSN1872-8332
AutoresPeter Grešner, Jolanta Gromadzińska, Ewa Jabłońska, Jacek Kaczmarski, Wojciech Wąsowicz,
Tópico(s)Glutathione Transferases and Polymorphisms
ResumoAbstract Aim of the study was to investigate the mRNA expression level of selenoprotein P ( SEPP1 ), 15-kDa selenoprotein ( SEP15 ) and glutathione peroxidase 1 ( hGPX1 ) in paired malignant and non-malignant tissue. To achieve this goal, the quantitative real-time PCR technique was utilized in paired tissue samples from 33 non-small cell lung cancer (NSCLC) patients. Simultaneously, the activity of glutathione peroxidases (GPX) and the level of thiobarbituric acid-reactive species (TBARS) in paired tissue specimens and the blood plasma selenium level was measured. We found significant down-regulation of SEPP1 expression level in tumorous lung tissue (2.732-fold; p <0.001). The expression of hGPX1 and SEP15 in tumorous tissue remained unchanged compared to healthy tissue. The level of TBARS in malignant tissue was significantly increased ( p <0.005) and negatively correlated with SEPP1 expression level ( R S =−0.3238; p <0.05). The activity of GPX in malignant tissue was significantly increased compared to the non-malignant one ( p <0.005) and negatively correlated with the expression level of SEPP1. It seems possible, that the down-regulation of SEPP1 expression may lead to an increased oxidative stress possibly resulting in lung carcinogenesis. Increased activity of GPX in tumorous lung tissue seems to be a feedback mechanism.
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