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Post-SELEX combinatorial optimization of aptamers

1997; Elsevier BV; Volume: 5; Issue: 6 Linguagem: Inglês

10.1016/s0968-0896(97)00044-8

1464-3391

Bruce E. Eaton, Larry Gold, Brian J. Hicke, Nebojša Janjié, Fiona M. Jucker, David P. Sebesta, Theodore M. Tarasow, Michael C. Willis, Dominic A. Zichi,

RNA and protein synthesis mechanisms

In vitro selection techniques provide a means of isolating nucleic acid ligands for binding to particular protein targets. Although most aptamers have quite high affinities for their target proteins, it has been shown that post-SELEX modification can result in further enhancement of binding affinity, as well as other desired properties. This has led to the current development of a more systematic approach to aptamer optimization using a combinatorial screening methodology.