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The MAGi RNA extraction method: a highly efficient and simple procedure for fresh and dry plant tissues

2008; Wiley; Volume: 89; Issue: 1 Linguagem: Inglês

10.1002/jsfa.3428

1097-0010

Ayşe Gül İnce, Mehmet Karaca,

Plant-Microbe Interactions and Immunity

Abstract BACKGROUND: Samples from different plant species, different organs or tissues at different times of the year, usually show great differences in their cell compositions, pH, and the endogenous RNase activities, decreasing the RNA yield and quality. RESULTS: In this study we describe a reagent and a simple total RNA isolation method for plant organs, tissues and dry seeds. The RNA extraction reagent (MAGi) is non‐toxic and can be stored at room temperature for several months to years. The principle of the total RNA extraction is that tissues are lysed in extraction solution with the aid of mortar homogenization–maceration, and cellular proteins, polysaccharides and DNA are removed from the RNA. We tested the reported method on more than 16 different types of plant seed and 15 different tissues and organs of pepper. CONCLUSION: The RNA extraction procedure reported in the present study greatly reduces the time required to isolate dry seed total RNA and other tissues by more than half as compared with the previously reported methods. The range of typical RNA yield and quality represents a significant improvement over existing protocols. The quality is high enough to be considered as suitable method for RT‐PCR, cDNA library construction and microarray gene expression studies. Copyright © 2008 Society of Chemical Industry