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The biosynthesis of nicotine from nicotinic acid: Chemical and radiochemical yields

1960; Elsevier BV; Volume: 91; Issue: 1 Linguagem: Inglês

10.1016/0003-9861(60)90467-7

1096-0384

Ray F. Dawson, David R. Christman, Marie L. Solt, Alfred P. Wolf,

Fungal and yeast genetics research

Chemical and radiochemical yields for the biosynthesis of nicotine from nicotinic acid-2-H3 as precursor have been investigated. Chemical yield was unaffected by additions to the experimental system of precursors of either the pyridine or the pyrrolidine rings. This effect did not result from rapid reduction of precursor concentration to rate-limiting levels by competing reactions. Radiochemical yields increased over a period of 30 days following single-batch addition of labeled precursor. Corrections for the radiochemical yield calculations of these and earlier experiments are derived to allow for (a) the duration of the incubation period, (b) the concentration of nicotinic acid in the culture fluids, and (c) the turnover rate of the labeled product.