Portal de Periódicos da CAPES

Genomic characterization of human astrovirus type 6 Katano virus and the establishment of a rapid and effective reverse transcription-polymerase chain reaction to detect all serotypes of human astrovirus

2000; Wiley; Volume: 61; Issue: 1 Linguagem: Inglês

10.1002/(sici)1096-9071(200005)61

1096-9071

Naomi Sakon, Kenji Yamazaki, Etsuko UTAGAWA, Yoshinobu Okuno, Isao Oishi,

Animal Virus Infections Studies

Journal of Medical VirologyVolume 61, Issue 1 p. 125-131 Genomic characterization of human astrovirus type 6 Katano virus and the establishment of a rapid and effective reverse transcription–polymerase chain reaction to detect all serotypes of human astrovirus Naomi Sakon, Corresponding Author Naomi Sakon [email protected] Laboratory of Virology, Osaka Prefectural Institute of Public Health, Osaka, JapanLaboratory of Virology, Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka 537–0025 Japan===Search for more papers by this authorKenji Yamazaki, Kenji Yamazaki Laboratory of Virology, Osaka Prefectural Institute of Public Health, Osaka, JapanSearch for more papers by this authorEtsuko Utagawa, Etsuko Utagawa Laboratory of Diarrhea, Department of Virology II, National Institute of Infectious Diseases, Tokyo, JapanSearch for more papers by this authorYoshinobu Okuno, Yoshinobu Okuno Laboratory of Virology, Osaka Prefectural Institute of Public Health, Osaka, JapanSearch for more papers by this authorIsao Oishi, Isao Oishi Laboratory of Pathology, Osaka Prefectural Institute of Public Health, Osaka, JapanSearch for more papers by this author Naomi Sakon, Corresponding Author Naomi Sakon [email protected] Laboratory of Virology, Osaka Prefectural Institute of Public Health, Osaka, JapanLaboratory of Virology, Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka 537–0025 Japan===Search for more papers by this authorKenji Yamazaki, Kenji Yamazaki Laboratory of Virology, Osaka Prefectural Institute of Public Health, Osaka, JapanSearch for more papers by this authorEtsuko Utagawa, Etsuko Utagawa Laboratory of Diarrhea, Department of Virology II, National Institute of Infectious Diseases, Tokyo, JapanSearch for more papers by this authorYoshinobu Okuno, Yoshinobu Okuno Laboratory of Virology, Osaka Prefectural Institute of Public Health, Osaka, JapanSearch for more papers by this authorIsao Oishi, Isao Oishi Laboratory of Pathology, Osaka Prefectural Institute of Public Health, Osaka, JapanSearch for more papers by this author First published: 31 March 2000 https://doi.org/10.1002/(SICI)1096-9071(200005)61:1 3.0.CO;2-BCitations: 23AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract We previously reported that human astrovirus type 6 (HAstV T6) was the etiologic agent of a large-scale outbreak of acute gastroenteritis that occurred in 1991 in Katano City, Osaka, Japan [Oishi et al., 1994]. The two representative strains, Katano virus K23 and K24, have been analyzed by sequencing the open reading frame 2 (ORF2) region after amplification by reverse transcription–polymerase chain reaction (RT-PCR). The ORF2 region of HAstV T6 strains, including K23, was found to be about 20 bp smaller than those of other types. There was 94% nucleotide sequence identity and 95% amino acid sequence identity between K23 and K24, with the Oxford strains belonging to HAstV T6. The high homology of the ORF2 region between the Katano and Oxford strains shows intratype genomic stability, irrespective of time and place of virus isolation. Comparing sequences of ORF2 of different HAstV serotypes, we established a rapid and highly sensitive detection system for HAstV types using RT-PCR with the AC230/AC1′ primer set designed from the 5′-terminal end region of ORF2. This RT-PCR system seems very useful in detecting at least two different viruses in a single PCR test tube using AC230/AC1′ in addition to the NV81/82, SM82 primer sets. Thus, our rapid and effective detection system may contribute to the epidemiologic characterization of astrovirus infections as well as Norwalk-like viruses. J. Med. Virol. 61:125–131, 2000. © 2000 Wiley-Liss, Inc. REFERENCES Appleton H, Higgins PG. 1975. Viruses and gastroenteritis in infants [Letter]. Lancet 1: 1297. Belliot G, Laveran H, Monroe SS. 1997. Outbreak of gastroenteritis in military recruits associated with serotype 3 astrovirus infection. J Med Virol 51: 101–106. Carter MJ, Willcocks MM. 1996. The molecular biology of astroviruses. Arch Virol [suppl] 12: 277–285. Glass RI, Noel J, Mitchell D, Herrmann JE, Blacklow NR, Pickering LK, Dennehy P, Ruiz-Palacios G, Guerrero ML, Monroe SS. 1996. The changing epidemiology of astrovirus-associated gastroenteritis: a review. Arch Virol [suppl] 12: 287–300. Jiang X, Wang J, Graham DY, Estes MK. 1992. Detection of Norwalk virus in stool by polymerase chain reaction. J Clin Microbiol 30: 2529–2534. Jonassen TO, Monceyron C, Lee TW, Kurtz JB, Grinde B. 1995. Detection of all serotypes of human astrovirus by the polymerase chain reaction. J Virol Meth 52: 327–334. Konno T, Suzuki H, Ishida N, Chiba R, Mochizuki K, Tsunoda A. 1982. Astrovirus-associated epidemic gastroenteritis in Japan. J Med Virol 9: 11–17. Koopmans MP, Bijen MH, Monroe SS, Vinje J. 1998. Age-stratified seroprevalence of neutralizing antibodies to astrovirus types 1 to 7 in humans in the Netherlands. Clin Diagn Lab Immunol 5: 33–37. Kriston S, Willcocks MM, Carter MJ, Cubitt WD. 1996. Seroprevalence of astrovirus type 1 and 6 in London, determined using recombinant virus antigen. Epidemiol Infect 117: 159–164. Kurtz JB, Lee TW. 1984. Human astrovirus serotypes. Lancet 2: 1405. Lee TW, Kurtz JB. 1981. Serial propagation of astrovirus in tissue culture with the aid of trypsin. J Gen Virol 57: 421–424. Lee TW, Kurtz JB. 1994. Prevalence of human astrovirus serotypes in the Oxford region 1976–92, with evidence for two new serotypes. Epidemiol Infect 112: 187–193. Madeley CR, Cosgrove BP. 1975. Twenty-eight nm particles in infantile gastroenteritis. Lancet 2: 451–452. Maldonado Y, Cantwell M, Old M, Hill D, Sanchez ML, Logan L, Millan-Velasco F, Valdespino JL, Sepulveda J, Matsui S. 1998. Population-based prevalence of symptomatic and asymptomatic astrovirus infection in rural Mayan infants. J Infect Dis 178: 334–339. Mitchell DK, Matson DO, Cubitt WD, Jackson LJ, Willcocks MM, Pickering LK, Carter MJ. 1999. Prevalence of antibodies to astrovirus types 1 and 3 in children and adolescents in Norfolk, Virginia. Pediatr Infect Dis J 18: 249–254. Moceyron C, Grinde B, Jonassen TO. 1997. Molecular characterization of the 3′-end of the astrovirus genome. Arch Virol 142: 699–706. Monroe SS, Glass RI, Noah N, Flewett TH, Caul EO, Ashton CI, Curry A, Field AM, Madeley R, Pead PJ. 1991. Electron microscopic reporting of gastrointestinal viruses in the United Kingdom, 1985–1987. J Med Virol 33: 193–198. Monroe SS, Jang B, Stine SE, Koopmans M, Glass RI. 1993. Subgenomic RNA sequence of human astrovirus supports classification of Astroviridae as a new family of RNA viruses. J Virol 67: 3611–3614. Mustafa H, Palombo EA, Bishop RF. 1998. Improved sensitivity of astrovirus-specific RT-PCR following culture of stool samples in CaCo-2 cells. J Clin Virol 11: 103–107. Myint S, Manley R, Cubitt D. 1994. Viruses in bathing waters. Lancet 343: 1640–1641. Noel JS, Lee TW, Kurtz JB, Glass RI, Monroe SS. 1995. Typing of human astroviruses from clinical isolates by enzyme immunoassay and nucleotide sequencing. J Clin Microbiol 33: 797–801. Oishi I, Yamazaki K, Kimoto T, Minekawa Y, Utagawa E, Yamazaki S, Inoue S, Grohmann GS, Monroe SS, Stine SE, Carcamo C, Ando T, Glass RI. 1994. A large outbreak of acute gastroenteritis associated with astrovirus among students and teachers in Osaka, Japan. J Infect Dis 170: 439–443. Prasad BVV, Hardy ME, Jiang X, Estes MK. 1996. Structure of Norwalk virus. Arch Virol 12: 237–242. Saito K, Ushijima H, Nishio O, Oseto M, Motohiro H, Ueda Y, Takagi M, Nakaya S, Ando T, Glass RI, Zaiman K. 1995. Detection of astrovirus from stool samples in Japan using reverse transcription and polymerase chain reaction amplification. Microbiol Immunol 39: 825–828. Sasaki Y, Ohta K, Hayashi Y, Sekine S, Hirata I, Sekine H, Itoh T. 1996. Diagnosis of viral gastroenteritis by detecting viral genomes with the use of reverse transcriptase–PCR. Ann Rep Tokyo Metropol Res Lab Public Health 47: 8–14. [Abstract in English]. Shastri S, Doane AM, Gonzales J, Upadhyayula U, Bass DM. 1998. Prevalence of astroviruses in children's hospital. J Clin Microbiol 36: 2571–2574. Traore O, Arnal C, Mignotte B, Maul A, Leveran H, Billaudel S, Schwartzbrod L. 1998. Reverse transcriptase PCR detection of astrovirus, hepatitis A virus, and poliovirus in experimentally contaminated mussels: comparison of several extraction and concentration methods. Appl Environ Microbiol 64: 3118–3122. Utagawa E, Nishizawa S, Sekine S, Hayashi Y, Ishihara Y, Oishi I, Iwasaki A, Yamashita I, Miyamura K, Yamazaki S, Inoue S, Glass RI. 1994. Astrovirus as a cause of gastroenteritis in Japan. J Clin Microbiol 32: 1841–1845. Wang J, Jiang X, Madore HP, Gray J, Desselberger U, Ando T, Seto Y, Oishi I, Lew JF, Green KY, Estes MK. 1994. Sequence diversity of small, round-structured viruses in the Norwalk virus group. J Virol 68: 5982–5990. Willcocks MM, Carter MJ, Laidler FR, Madeley CR. 1990. Growth and characterization of human fecal astrovirus cell line. Arch Virol 113: 73–81. Willcocks MM, Kurtz JB, Lee TW, Carter MJ. 1995. Prevalence of human astrovirus serotype 4: capsid protein sequence and comparison with other strains. Epidemiol Infect 114: 385–391. Citing Literature Volume61, Issue1May 2000Pages 125-131 ReferencesRelatedInformation